入侵植物Wedeliatrilobata在各个方面都受益,如营养吸收和环境适应性,通过与丛枝菌根真菌(AMF)建立密切的共生关系。然而,我们对AMF是否可以通过影响其代谢谱而使W.三叶获益的理解仍然有限.在这项研究中,采用液相色谱-串联质谱(LC-MS/MS)对AMF接种条件下的三叶草代谢产物进行分析。代谢组学分析鉴定了接种AMF和未接种AMF的组之间的119种差异表达的代谢物(DEM)。与没有接种AMF的植物相比,接种AMF的植物显示69种代谢物的相对表达上调,50种代谢物的相对表达下调。AMF显着增加植物中各种初级和次级代谢产物的水平,包括氨基酸,有机酸,植物激素,黄酮类化合物,和其他人,氨基酸是鉴定物质中最丰富的。鉴定的DEM绘制了53条代谢途径,有7条途径受到AMF的强烈影响,特别是苯丙氨酸代谢途径。此外,我们还观察到AMF在三叶W.显着促进这种植物的芽生长。这些代谢产物和代谢途径的变化显著影响植物的多种生理生化过程,如自由基清除,渗透调节,细胞结构稳定性,和材料合成。总之,AMF重新编程了W.trilobata的代谢途径,导致初级和次级代谢组的变化,从而有利于W.trilobata的生长,并增强其应对各种生物和非生物胁迫的能力。这些发现阐明了AMF在入侵植物W.trilobata中的分子调节作用,并为其竞争和抗逆机制的研究提供了新的见解。
The invasive plant Wedelia trilobata benefits in various aspects, such as nutrient absorption and environmental adaptability, by establishing a close symbiotic relationship with arbuscular mycorrhizal fungi (AMF). However, our understanding of whether AMF can benefit W. trilobata by influencing its metabolic profile remains limited. In this study, Liquid chromatography-tandem mass spectrometry (LC-MS/MS) was conducted to analyze the metabolites of W. trilobata under AMF inoculation. Metabolomic analysis identified 119 differentially expressed metabolites (DEMs) between the groups inoculated with AMF and those not inoculated with AMF. Compared to plants with no AMF inoculation, plants inoculated with AMF showed upregulation in the relative expression of 69 metabolites and downregulation in the relative expression of 50 metabolites. AMF significantly increased levels of various primary and secondary metabolites in plants, including amino acids, organic acids, plant hormones, flavonoids, and others, with amino acids being the most abundant among the identified substances. The identified DEMs mapped 53 metabolic pathways, with 7 pathways strongly influenced by AMF, particularly the phenylalanine metabolism pathway. Moreover, we also observed a high colonization level of AMF in the roots of W. trilobata, significantly promoting the shoot growth of this plant. These changes in metabolites and metabolic pathways significantly affect multiple physiological and biochemical processes in plants, such as free radical scavenging, osmotic regulation, cell structure stability, and material synthesis. In summary, AMF reprogrammed the metabolic pathways of W. trilobata, leading to changes in both primary and secondary metabolomes, thereby benefiting the growth of W. trilobata and enhancing its ability to respond to various biotic and abiotic stressors. These findings elucidate the molecular regulatory role of AMF in the invasive plant W. trilobata and provide new insights into the study of its competitive and stress resistance mechanisms.