禾谷镰刀菌,一种毁灭性的真菌病原体,给全球农作物产量造成巨大的经济损失。本研究基于植物促进生长和抗性诱导试验,研究了早链霉菌S10减轻小麦幼苗中谷草芽孢杆菌胁迫的潜力。生物测定表明,S10表现出多种植物生长促进特性,包括铁载体的生产,1-氨基环丙烷-1-羧酸脱氨酶(ACC),和吲哚-3-乙酸(IAA),磷酸盐溶解,和固氮。同时,盆栽试验表明S10促进小麦植株发育,大幅提高小麦高度,体重,根系活动,和叶绿素含量。始终如一,基因组挖掘确定了丰富的与植物生长促进相关的基因。S10诱导小麦幼苗对谷草的抗性。S10预处理小麦幼苗的发病率和病情指数分别降低了近52%和65%,分别,与仅在非接触接种试验中感染禾谷赤霉病的人相比。此外,S10增强了call的沉积和活性氧(ROS)的积累,并诱导了CAT的活性,SOD,POD,PAL,和PPO。此外,实时定量PCR(qRT-PCR)结果表明,S10预处理增加了SA-(PR1.1,PR2,PR5和PAL1)和JA/ET相关基因(PR3,PR4a,PR9和PDF1.2)在小麦幼苗中感染。总之,S.pratensisS10可能是小麦幼苗疫病管理和植物生产力提高中的集成生物制剂和生物肥料。
Fusarium graminearum, a devastating fungal pathogen, causes great economic losses to crop yields worldwide. The present study investigated the potential of Streptomyces pratensis S10 to alleviate F. graminearum stress in wheat seedlings based on plant growth-promoting and resistance-inducing assays. The bioassays revealed that S10 exhibited multiple plant growth-promoting properties, including the production of siderophores, 1-aminocyclopropane-1-carboxylic acid deaminase (ACC), and indole-3-acetic acid (IAA), phosphate solubilization, and nitrogen fixation. Meanwhile, the pot experiment demonstrated that S10 improved wheat plant development, substantially enhancing wheat height, weight, root activity, and chlorophyll content. Consistently, genome mining identified abundant genes associated with plant growth promotion. S10 induced resistance against F. graminearum in wheat seedlings. The disease incidence and disease index reduced by nearly 52% and 65% in S10 pretreated wheat seedlings, respectively, compared with those infected with F. graminearum only in the non-contact inoculation assay. Moreover, S10 enhanced callose deposition and reactive oxygen species (ROS) accumulation and induced the activities of CAT, SOD, POD, PAL, and PPO. Furthermore, the quantitative real-time PCR (qRT-PCR) results indicated that S10 pretreatment increased the expression of SA- (PR1.1, PR2, PR5, and PAL1) and JA/ET-related genes (PR3, PR4a, PR9, and PDF1.2) in wheat seedlings upon F. graminearum infection. In summary, S. pratensis S10 could be an integrated biological agent and biofertilizer in wheat seedling blight management and plant productivity enhancement.