在黑暗和光照条件下,测试了9个钌CNC钳配合物(1-9)在细胞培养物中的抗癌活性。这些配合物包括各种CNC钳配体,包括OH,OMe,或甲基(Me)变化的吡啶基环和翼尖N-杂环卡宾(NHC)基团上的Me取代基,苯基(Ph),mesityl(Mes),和2,6-二异丙基苯基(Dipp)。支持配体包括乙腈,Cl,和2,2'-联吡啶(bpy)供体。本文描述了配合物8和9的合成,并通过光谱(1HNMR,IR,UV-Vis,MS)和分析技术。本文报道了8和9的单晶X射线衍射结果。其它复合物(1-7)在别处报道。四种最亲脂性的钌配合物(6、7、8和9)显示出最佳的活性。MCF7癌细胞具有显示细胞毒性的复合物6和9和显示光活化的光细胞毒性的复合物7和8。这些化合物在辛醇和水之间的分布记录为log(Do/w)值,和增加的log(Do/w)值与改善的活动与癌细胞。总的来说,亲脂性翼尖基团(例如Ph,Mes,Dipp)在NHC环上和较低的阳离子电荷(1与2+)似乎有利于提高抗癌活性。
Nine ruthenium CNC pincer complexes (1-9) were tested for anticancer activity in cell culture under both dark and light conditions. These complexes included varied CNC pincer ligands including OH, OMe, or Me substituents on the pyridyl ring and wingtip N-heterocyclic carbene (NHC) groups which varied as methyl (Me), phenyl (Ph), mesityl (Mes), and 2,6-diisopropylphenyl (Dipp). The supporting ligands included acetonitrile, Cl, and 2,2\'-bipyridine (bpy) donors. The synthesis of complexes 8 and 9 is described herein and are fully characterized by spectroscopic (1H NMR, IR, UV-Vis, MS) and analytical techniques. Single crystal X-ray diffraction results are reported herein for 8 and 9. The other complexes (1-7) are reported elsewhere. The four most lipophilic ruthenium complexes (6, 7, 8, and 9) showed the best activity vs. MCF7 cancer cells with complexes 6 and 9 showing cytotoxicity and complex 7 and 8 showing light activated photocytotoxicity. The distribution of these compounds between octanol and water is reported as log(Do/w) values, and increasing log(Do/w) values correlate roughly with improved activity vs. cancer cells. Overall, lipophilic wingtip groups (e.g. Ph, Mes, Dipp) on the NHC ring and a lower cationic charge (1+ vs. 2+) appears to be beneficial for improved anticancer activity.