■由于生理和基因组问题,甘薯面临育种挑战。γ辐射是一种诱导作物遗传变异的新方法。与野生型“Tongchaeru”品种相比,我们分析了伽马射线诱导的甘薯突变体茎发育改变的转录组变化。
进行RNA测序分析以鉴定与茎发育相关的基因表达的变化。
■转录组分析确定了8,931个上调的基因和6,901个下调的基因,包括生长素响应小辅助剂RNA(SAUR)和三个植物色素相互作用因子4(PIF4)基因的上调。PIF4对于调节拟南芥早期生长素响应SAUR基因的表达和茎的生长至关重要。在突变体中,与茎伸长有关的几个基因,包括PIF4和参与各种信号通路如生长素和赤霉素,被上调了。
■我们的结果表明,伽马射线诱导的突变通过调节涉及PIF4和SAUR基因表达的复杂调控网络来影响生长素依赖性茎发育,和其他信号通路,如赤霉素和乙烯信号基因。这项研究增强了我们对甘薯茎生长的调节机制的理解,为基因组学辅助育种工作提供有价值的见解。
UNASSIGNED: Sweetpotato faces breeding challenges due to physiological and genomic issues. Gamma radiation is a novel approach for inducing genetic variation in crops. We analyzed the transcriptomic changes in gamma ray-induced sweetpotato mutants with altered stem development compared with those in the wild-type \'Tongchaeru\' cultivar.
UNASSIGNED: RNA sequencing analyses were performed to identify changes in the expression of genes related to stem development.
UNASSIGNED: Transcriptomic analysis identified 8,931 upregulated and 6,901 downregulated genes, including the upregulation of the auxin-responsive SMALL AUXIN UP RNA (SAUR) and three PHYTOCHROME INTERACTING FACTOR 4 (PIF4) genes. PIF4 is crucial for regulating the expression of early auxin-responsive SAUR genes and stem growth in Arabidopsis thaliana. In the mutant, several genes related to stem elongation, including PIF4 and those involved in various signaling pathways such as auxin and gibberellin, were upregulated.
UNASSIGNED: Our results suggest that gamma ray-induced mutations influence auxin-dependent stem development by modulating a complex regulatory network involving the expression of PIF4 and SAUR genes, and other signaling pathways such as gibberellin and ethylene signaling genes. This study enhances our understanding of the regulatory mechanisms underlying stem growth in sweetpotato, providing valuable insights for genomics-assisted breeding efforts.