在这项研究中,通过生物信息学分析和基因组数据鉴定了菲律宾蛤仔的Fox基因家族。结果表明,在菲律宾R。中共鉴定出21个Fox基因,分为16个亚科,包括Foxa亚家族的两个成员(Foxa1,Foxa2),Foxl亚家族的三个成员(Foxl1b,Foxl1a,FOXL2),Foxn亚家族的三个成员(FOXN3,FOX4A,Foxn4b)和其他家庭的一名成员。染色体分布,域,保守的图案,内含子,预测了这21个Fox基因的外显子和蛋白质三级结构。通过分析菲律宾念珠菌的RNA-seq数据,发现Fox基因家族在不同组织中差异表达,不同的发育阶段和热和冷胁迫下。大多数Fox基因在四个组织中高表达:唇瓣,性腺,刺和脚。大多数Fox基因在囊胚期高度表达。年夜多半Fox基因在两个群体的高温组中高表达,还有Foxo,FOXG1在低温组中高表达。此外,qPCR结果显示,在急性冷应激下,Foxo和Foxj1b基因的表达水平显著升高。因此,我们推测Fox基因可能在菲律宾R.philippinarum的胚胎发育和温度胁迫中起重要作用,本研究为进一步探索Fox介导低温耐受的分子机制提供了依据。
In this study, the Fox gene family of Ruditapes philippinarum was identified by bioinformatics analysis and genome data. The results showed that a total of 21 Fox genes were identified in R. philippinarum, which were divided into 16 subfamilies, including two members of Foxa subfamily (Foxa1, Foxa2), three members of Foxl subfamily (Foxl1b, Foxl1a, FOXL2), three members of Foxn subfamily (FOXN3, FOX4A, Foxn4b) and one member of other families. The chromosome distribution, domains, conserved motifs, introns, exons and protein tertiary structures of these 21 Fox genes were predicted. By analyzing the RNA-seq data of R. philippinarum, it was found that the Fox gene family was differentially expressed in different tissues, different developmental stages and under heat and cold stress. Most of Fox genes were highly expressed in four tissues: labial palp, gonad, gill and foot. Most of the Fox genes were highly expressed in blastula stage. Most of the Fox genes were highly expressed in high temperature group of two populations, and Foxo, FOXG1 were highly expressed in low temperature group. In addition, qPCR showed that the expression levels of Foxo and Foxj1b genes increased significantly under acute cold stress. Therefore, we speculate that Fox genes may play important roles in embryo development and the temperature stress of R. philippinarum, and this study provides a basis for further exploring the molecular mechanism of low temperature tolerance mediated by Fox.