尽管据报道,可溶性硅酸盐通过促进木质素或游离脂肪酸的沉积来加速甜瓜果实的伤口愈合,硅酸钠是否影响生物合成,马铃薯伤口愈合过程中Suberin单体的交联和运输仍然未知。在这项研究中,硅酸钠上调4-香豆酸:辅酶A连接酶(4CL)的表达和活性,苯丙氨酸解氨酶(PAL),并促进酚酸(咖啡酸,对香豆酸,肉桂酸,芥子酸,和阿魏酸)在块茎伤口中。同时,硅酸钠上调甘油-3-磷酸酰基转移酶(StGPAT)的表达,脂酰还原酶(StFAR),长链酰基辅酶A合成酶(StLACS),β-酮脂酰辅酶A合酶(StKCS),和细胞色素P450(StCYP86A33),从而增加了α的水平,ω-二酸,ω-羟基酸,和伤口中的原醇。硅酸钠还诱导ω-羟基酸/脂肪醇羟基肉桂酰转移酶(StFHT)的表达,ABC运输机(StABCG),并促进了伤口表面的Suberin沉积,从而降低块茎疾病指数和愈合过程中的体重减轻。一起来看,硅酸钠可能通过诱导类苯丙素途径和脂肪酸代谢来加速马铃薯块茎伤口的针叶素积累。
Although soluble silicate was reported to accelerate wound healing in muskmelon fruit through encouraging the deposition of lignin or free fatty acids, whether sodium silicate affects the biosynthesis, cross-linking and transport of suberin monomers during potato wound healing remains unknown. In this study, sodium silicate upregulated the expression and activity of 4-coumarate: coenzyme A ligase (4CL), phenylalanine ammonia lyase (PAL), and promoted the synthesis of phenolic acids (caffeic acid, p-coumaric acid, cinnamic acid, sinapic acid, and ferulic acid) in tuber wounds. Meanwhile, sodium silicate upregulated the expression of glycerol-3-phosphate acyltransferase (StGPAT), fatty acyl reductase (StFAR), long-chain acyl-CoA synthetase (StLACS), β-ketoacyl-CoA synthase (StKCS), and cytochrome P450 (StCYP86A33), and thus increased the levels of α, ω-diacids, ω-hydroxy acids, and primary alcohols in wounds. Sodium silicate also induced the expression of ω-hydroxy acid/fatty alcohol hydroxycinnamoyl transferase (StFHT), ABC transporter (StABCG), and promoted the deposition of suberin in wound surface, hence reducing tuber disease index and weight loss during healing. Taken together, sodium silicate may accelerate suberin accumulation at potato tubers wound through inducing the phenylpropanoid pathway and fatty acid metabolism.