基因组的计算注释和当前代谢模型的预测潜力的进展,基于数千种实验表型,允许它们应用于在分类单元内的生态生理学分化水平上识别代谢途径的多样性并预测表型,次生代谢产物,主机关联的交互,生存能力,和拟议环境条件下的生化生产力。海洋细菌物种Pseudoalteromonasdistributa成员的显着独特表型以及无法使用通用分子标记,因此,如果没有基因组规模分析和代谢重建,就无法在Pseudoalteromonas属中鉴定它们并预测其生物技术潜力。一种新的菌株,KMM6257,类胡萝卜素表型,与一种习以为常的海星隔离,修改了P.distrarta的描述,特别是在4至37°C的温度增长范围内。系统基因组学阐明了所有可用的密切相关物种的分类状态。P.tardita具有推定的甲基赤藓糖醇磷酸途径II和4,4'-二聚二戊酸生物合成,与C30类胡萝卜素有关,和它们的功能类似物,芳基多烯生物合成基因簇(BGC)。然而,某些菌株中的黄橙色色素沉着表型与编码用间苯二酚酯化的芳基多烯的杂种BGC的存在一致。藻酸盐降解和糖基化免疫抑制剂的生产,类似于巴西石素,链球菌,和核苷,是共同的预测特征。淀粉,琼脂,角叉菜胶,木糖,木质素衍生的化合物降解,多糖,叶酸,和钴胺素生物合成都是菌株特异性的。
Advances in the computational annotation of genomes and the predictive potential of current metabolic models, based on more than thousands of experimental phenotypes, allow them to be applied to identify the diversity of metabolic pathways at the level of ecophysiology differentiation within taxa and to predict phenotypes, secondary metabolites, host-associated interactions, survivability, and biochemical productivity under proposed environmental conditions. The significantly distinctive phenotypes of members of the marine bacterial species Pseudoalteromonas distincta and an inability to use common molecular markers make their identification within the genus Pseudoalteromonas and prediction of their biotechnology potential impossible without genome-scale analysis and metabolic reconstruction. A new strain, KMM 6257, of a carotenoid-like phenotype, isolated from a deep-habituating starfish, emended the description of P. distincta, particularly in the temperature growth range from 4 to 37 °C. The taxonomic status of all available closely related species was elucidated by phylogenomics. P. distincta possesses putative methylerythritol phosphate pathway II and 4,4\'-diapolycopenedioate biosynthesis, related to C30 carotenoids, and their functional analogues, aryl polyene biosynthetic gene clusters (BGC). However, the yellow-orange pigmentation phenotypes in some strains coincide with the presence of a hybrid BGC encoding for aryl polyene esterified with resorcinol. The alginate degradation and glycosylated immunosuppressant production, similar to brasilicardin, streptorubin, and nucleocidines, are the common predicted features. Starch, agar, carrageenan, xylose, lignin-derived compound degradation, polysaccharide, folate, and cobalamin biosynthesis are all strain-specific.