Oroxylum(L.)Kurz对抗炎症,细菌,和氧化已经有了很好的记录。此外,它通常作为茶食用。然而,其主要化合物的体内机制尚未得到很好的阐明。在这项研究中,建立了一种高度选择性和灵敏的UHPLC-Q-TOF-MS方法,结合基于质谱的正交投影(MSOP)理论和四步分析策略,并对其进行了验证,以鉴定大鼠口服Oroxylum后的代谢物(L.)库尔兹摘录。此外,建立了灵敏的LC-MS/MS方法,并首次验证了10种主要黄酮类化合物在大鼠体内的药代动力学分析。值得注意的是,在血液中总共鉴定出47种代谢物,胆汁,尿液,和粪便样本.oroxinA的最大血浆浓度(Cmax)值,oroxinB,黄芩苷,chrysin,黄芩素,scutellarein,芹菜素,槲皮素和异鼠李素为2945.1±11.23ng/mL,3123.9±16.37ng/mL,130.40±27.52ng/mL,117.20±28.54ng/mL,64.12±19.33ng/mL,97.22±24.27ng/mL,145.22±29.92ng/mL,45.19±18.84ng/mL,67.32±15.78ng/mL和128.44±26.42ng/mL。芹菜素的给药时间曲线出现双峰,由于肝肠再循环。本研究表明,MSOP方法为复杂体系中黄酮类代谢物的鉴定提供了比传统方法更多的技术支持。
The pharmacological effects of flavonoids in Oroxylum indicum (L.) Kurz against inflammation, bacterial, and oxidation have been well-documented. Additionally, it is commonly consumed as tea. However, the in vivo mechanism of its main compounds has not been well elucidated. In this study, a highly selective and sensitive UHPLC-Q-TOF-MS method combined with Mass Spectrum-based Orthogonal Projection (MSOP) theory and four-step analytical strategy was established and validated to identify metabolites in rats following oral administration Oroxylum indicum (L.) Kurz extract. Furthermore, a sensitive LC-MS/MS method was developed and validated for the first time to analyze the pharmacokinetics of ten main flavonoids in rats. Notably, a total of 47 metabolites were identified in blood, bile, urine, and feces samples. The maximum plasma concentration (Cmax) values for oroxin A, oroxin B, baicalin, chrysin, baicalein, scutellarein, apigenin, quercetin oroxylin A and isorhamnetin were 2945.1 ± 11.23 ng/mL, 3123.9 ± 16.37 ng/mL, 130.40 ± 27.52 ng/mL, 117.20 ± 28.54 ng/mL, 64.12 ± 19.33 ng/mL, 97.22 ± 24.27 ng/mL, 145.22 ± 29.92 ng/mL, 45.19 ± 18.84 ng/mL, 67.32 ± 15.78 ng/mL and 128.44 ± 26.42 ng/mL. A double peak was observed in the drug-time curve of apigenin, due to enterohepatic recirculation. This study demonstrated that MSOP method provided more technical support for the identification of flavonoid metabolites in complex system than traditional methods.