在这项研究中,我们重组表达了小反刍动物病毒（PPRV）的V蛋白，并使用间接ELISA（i-ELISA）评估了其对PPRV感染的诊断价值。在1：400的血清稀释度下，V蛋白的包被抗原的最佳浓度为15ng/孔，最佳阳性阈值为0.233。交叉反应性分析表明，基于V蛋白的i-ELISA对PPRV具有特异性，具有一致的可重复性，并且在病毒中和测试中显示出82.6％的特异性和100％的灵敏度。在ELISA中使用重组V蛋白作为抗原可用于PPRV感染的血清流行病学研究。
In this study, we recombinantly expressed the V protein of the peste des petits ruminants virus (PPRV) and evaluated its diagnostic value for PPRV infection using an indirect ELISA (i-ELISA). The optimal concentration of the coated antigen of V protein was 15 ng/well at a serum dilution of 1:400, and the optimal positive threshold value was 0.233. A cross-reactivity assay showed that the V protein-based i-ELISA was specific to PPRV with consistent reproducibility and showed a specificity of 82.6% and a sensitivity of 100% with a virus neutralization test. Using the recombinant V protein as an antigen in ELISA is useful for seroepidemiological studies of PPRV infections.
