■响应于生物膜形成的牙龈炎可能表现出不同的轨迹。本研究的目的是表征不同牙龈炎模式的健康个体的牙龈上微生物群和唾液细胞因子和蛋白质水平的组成。为了检验这一假设,牙龈炎的表现与牙龈上微生物群的具体概况相关,唾液细胞因子,和蛋白质。
40名口腔和全身健康的人在14天内不接受所有口腔卫生程序,然后是14天的定期口腔护理的解决期。记录牙龈上斑块水平和探查出血(BOP),并在基线时收集牙龈上斑块和唾液样本,第14天和第28天。基于从基线到第14天的BOP%变化,快速(n=15),中等(n=10),和慢(n=15)反应者被确定。牙龈上微生物群组成,唾液细胞因子,和蛋白质水平在基线时进行组间比较,第14天和第28天。
■Capnocytophaga的基线丰度明显更高,Eikenella,并且在快速反应者中记录了弯曲杆菌的种类,而在慢反应者中检测到明显较高的基线链球菌丰度.反应迟钝的人表现出高度的韧性,在基线和14天(第28天)后,微生物组成差异最小。相反,核心微生物群成员的相对丰度存在显著差异,链球菌,放线菌,和Rothia物种,在基线样本中与第28天样本相比,在快速反应者中注意到。在所有组中记录相当的基线细胞因子和蛋白质水平。
■牙龈上微生物群组成,但不是唾液细胞因子和蛋白质谱,似乎会影响全身健康个体牙龈炎发展过程中炎症反应的程度。
牙龈上微生物群的基线组成可能预测不同的牙龈炎轨迹。牙龈炎后的微生物弹性可能会增强牙龈炎轨迹缓慢的个体的口腔稳态。
UNASSIGNED: Gingivitis in response to biofilm formation may exhibit different trajectories. The purposes of the present study were to characterize the composition of the supragingival microbiota and salivary cytokine and protein levels in healthy individuals with different gingivitis patterns, to test the hypothesis that manifestations of gingivitis associate with specific profiles in terms of supragingival microbiota, salivary cytokines, and proteins.
UNASSIGNED: Forty orally and systemically healthy individuals refrained from all oral hygiene procedures for a period of 14 days, followed by a resolution period of 14 days with regular oral care. Supragingival plaque level and bleeding on probing (BOP) were recorded, and supragingival plaque as well as saliva samples were collected at baseline, day 14, and day 28. Based on change in BOP% from baseline to day 14, rapid (n = 15), moderate (n = 10), and slow (n = 15) responders were identified. Supragingival microbiota composition, salivary cytokine, and protein levels were compared between groups at baseline, day 14, and day 28.
UNASSIGNED: A significantly higher baseline abundance of Capnocytophaga, Eikenella, and Campylobacter species were recorded in rapid responders, whereas a significantly higher baseline abundance of Streptococcus species were detected in slow responders. Slow responders expressed a high degree of resilience, with minimal difference in microbial composition at baseline and after 14 days of resolution (day 28). On the contrary, significant differences in relative abundance of members of the core microbiota, Streptococcus, Actinomyces, and Rothia species, was noted in baseline samples versus day 28 samples in rapid responders. Comparable baseline cytokine and protein levels were recorded in all groups.
UNASSIGNED: Supragingival microbiota composition, but not saliva cytokine and protein profiles, seems to influence the extent of the inflammatory response during development of gingivitis in systemically healthy individuals.
Baseline composition of the supragingival microbiota might predict different gingivitis trajectories.Microbial resilience after gingivitis might augment oral homeostasis in individuals with a slow gingivitis trajectory.