背景:腹膜内粘连形成是手术后的一个重要问题,导致重大的临床和经济后果。甘草具有几种药理特性,包括抗炎,抗微生物,抗氧化剂,抗癌,和免疫调节活动。
目标:因此,我们旨在研究G.glabra对大鼠术后腹腔粘连发展的影响。
方法:体重200~250g的雄性Wistar大鼠分为6组(n=8):第1组:正常组(非手术组),手术组包括第2组:对照组接受赋形剂,第三组:G.glabra0.5%w/v,第4组:G.glabra1%w/v,第5组:G.glabra2%w/v,第6组:地塞米松,0.4%w/v在盲肠的一侧使用软灭菌砂纸进行腹内粘连,并用2ml提取物或媒介物轻微洗涤腹膜。此外,粘附评分和炎症介质水平的宏观检查[干扰素(IFN)-γ,前列腺素E2(PGE2)],纤维化标志物[白细胞介素(IL)-4,转化生长因子(TGF)-],和氧化因素[丙二醛(MDA),一氧化氮代谢物(NO),和还原型谷胱甘肽(GSH)]进行了评估。还对小鼠成纤维细胞L929和NIH/3T3细胞系进行了体外毒性。
结果:我们发现更高的粘附水平(P<0.001),IFN-γ(P<0.001),PGE2(P<0.001),IL-4(P<0.001),TGF-β(P<0.001),MDA(P<0.001),和NO(P<0.001),对照组GSH水平较低(P<0.001)。相比之下,G.glabra浓度依赖性和地塞米松减轻了粘连水平(P<0.05),炎症介质(P<0.001-0.05),纤维化(P<0.001-0.05),和氧化(P<0.001-0.05)因素,与对照组相比,同时传播抗氧化标记(P<0.001-0.05)。结果还表明,提取物在300µg/ml以下时没有显着降低细胞活力(P>0.05)。
结论:G.glabra可以通过其抗炎作用浓度依赖性地减轻腹膜粘连的形成,抗纤维化,和抗氧化性能。然而,需要进一步的临床研究才能批准G.glabra可能是预防术后粘连并发症的有希望的候选药物.
BACKGROUND: Intraperitoneal adhesion formation is a significant problem following surgeries, resulting in substantial clinical and economic consequences. Glycyrrhiza glabra has several pharmacological properties consisting of anti-inflammatory, anti-microbial, anti-oxidant, anti-cancer, and immunomodulatory activities.
OBJECTIVE: Therefore, we aimed to investigate the impacts of G. glabra on the development of post-operative abdominal adhesion in a rat model.
METHODS: Male Wistar rats weighing 200-250 g were divided into six groups (n = 8): Group 1: normal group (non-surgical), and the surgical groups including Group 2: control group received the vehicle, Group 3: G. glabra 0.5% w/v, Group 4: G. glabra 1% w/v, Group 5: G. glabra 2% w/v, and Group 6: dexamethasone, 0.4% w/v. The intra-abdominal adhesion was performed utilizing soft sterilized sandpaper on one side of the cecum, and the peritoneum was slightly washed with 2 ml of the extract or vehicle. In addition, macroscopic examination of adhesion scoring and the levels of inflammatory mediators [interferon (IFN)-γ, prostaglandin E2 (PGE2)], fibrosis markers [interleukin (IL)-4, transforming growth factor (TGF)-ꞵ], and oxidative factors [malondialdehyde (MDA), nitric oxide metabolites (NO), and reduced glutathione (GSH)] were evaluated. In vitro toxicities were also done on mouse fibroblast L929 and NIH/3T3 cell lines.
RESULTS: We found higher levels of adhesion (P < 0.001), IFN-γ(P < 0.001), PGE2(P < 0.001), IL-4(P < 0.001), TGF-β(P < 0.001), MDA(P < 0.001), and NO(P < 0.001), and lower levels of GSH(P < 0.001) in the control group. In contrast, G. glabra concentration dependent and dexamethasone alleviated the levels of adhesion (P < 0.05), inflammatory mediators (P < 0.001-0.05), fibrosis (P < 0.001-0.05), and oxidative (P < 0.001-0.05) factors, while propagating the anti-oxidant marker (P < 0.001-0.05) in comparison to the control group. Results also showed that the extract did not significantly reduce cell viability up to 300 µg/ml (P > 0.05).
CONCLUSIONS: G. glabra could concentration-dependently mitigate peritoneal adhesion formation through its anti-inflammatory, anti-fibrosis, and anti-oxidant properties. However, further clinical investigations are required to approve that G. glabra may be a promising candidate against post-surgical adhesive complications.