■连作影响了土壤酶活性的稳定性和微生物群落的结构特征。由于复杂根际微生物群落研究的挑战,农田生态系统中这些微生物群落的组成和功能仍然难以捉摸。这里,我们研究了不同连作年限酿酒葡萄根际微生物群落,并探讨了其与土壤酶活性的关系。
■在一个未耕种的荒地和四个葡萄园的根际土壤上进行了宏基因组测序,连续种植时间不同。
■主要微生物是细菌(98.39%),其次是古生菌(1.15%)和真核生物(0.45%)。连续种植导致根瘤菌和微球藻的相对丰度显着增加,但杆菌属细菌的显着减少。在属一级,75、88、65、132和128个微生物属是未开垦荒地特有的,连续种植5年、10年、15年和20年,分别。具有信号转导功能的基因的相对丰度最高。在这项研究中测量的所有酶的活性在连续种植5年时达到峰值,然后随着10到15年的连作而减少,但又增加了20年。此外,土壤酶活性,特别是碱性磷酸酶在属水平上与优势微生物的多样性显着相关。此外,偶联酶活性对微生物群落多样性的影响大于单个酶。
■我们的发现揭示了土壤微生物群落和酶活性的组成和功能,以响应种植年份的变化,这对克服连作障碍和优化土地利用具有重要意义。
UNASSIGNED: Continuous cropping affected the stability of soil enzyme activity and the structural characteristics of microbial community. Owing to challenges in the study of complex rhizosphere microbial communities, the composition and function of these microbial communities in farmland ecosystems remain elusive. Here, we studied the microbial communities of the rhizosphere of wine grapes with different years of continuous cropping and investigated their relationships with soil enzyme activity.
UNASSIGNED: Metagenomic sequencing was conducted on the rhizosphere soils from one uncultivated wasteland and four vineyards with varying durations of continuous cropping.
UNASSIGNED: The predominant microbial were bacteria (98.39%), followed by archaea (1.15%) and eukaryotes (0.45%). Continuous cropping caused a significant increase in the relative abundance of Rhizobiales and Micrococcales but a marked decrease in Solirubrobacterales. At the genus level, 75, 88, 65, 132, and 128 microbial genera were unique to uncultivated wasteland, 5, 10, 15, and 20 years of continuous cropping, respectively. The relative abundance of genes with signal transduction function was the highest. The activity of all enzymes measured in this study peaked at 5 years of continuous cropping, and then decreased with 10 to 15 year of continuous cropping, but increased at 20 years again. In addition, soil enzyme activity, especially of alkaline phosphatase was significantly correlated with the diversity of the dominant microorganisms at the genus level. Moreover, the coupled enzyme activities had a greater impact on the diversity of the microbial community than that of individual enzymes.
UNASSIGNED: Our findings reveal the composition and function of the soil microbial communities and enzymes activity in response to changes in cropping years, which has important implications for overcoming continuous cropping obstacles and optimizing land use.