Fmoc-二苯丙氨酸(Fmoc-FF)是低分子量肽水凝胶剂。这种简单的全芳族肽可以产生自支撑的水凝胶材料,已被提出作为诊断和药物应用的新型材料。我们对Fmoc-FF聚集的分子决定子的知识被用作设计新的基于肽的胶凝剂的指导,具有用于开发改进工具的功能。这里,通过研究Fmoc-FFK三肽的性质,我们扩大了基于Fmoc-FF的水凝胶基质的过多,单独或与Fmoc-FF组合。对于多组分矩阵,Fmoc-FFK和Fmoc-FF之间的相对重量比(具体来说,评价1/1、1/5、1/10和1/20w/w)。使用不同的实验技术研究了所有系统及其多尺度组织,包括流变学,圆二色性,傅里叶变换红外光谱,和扫描电子显微镜(SEM)。还通过在HaCaT和3T3-L1细胞系上进行体外测试来描述所研究系统的生物相容性的初步概况。此外,在Fmoc-FF部分的C末端的赖氨酸(K)残基引入超分子材料化学官能团(氨基),其可用于修饰/衍生感兴趣的生物活性分子,包括诊断探针,螯合剂,活性药物成分,或肽核酸。
Fmoc-diphenylalanine (Fmoc-FF) is a low-molecular-weight peptide hydrogelator. This simple all-aromatic peptide can generate self-supporting hydrogel materials, which have been proposed as novel materials for diagnostic and pharmaceutical applications. Our knowledge of the molecular determinants of Fmoc-FF aggregation is used as a guide to design new peptide-based gelators, with features for the development of improved tools. Here, we enlarge the plethora of Fmoc-FF-based hydrogelated matrices by studying the properties of the Fmoc-FFK tripeptide, alone or in combination with Fmoc-FF. For multicomponent matrices, the relative weight ratios between Fmoc-FFK and Fmoc-FF (specifically, 1/1, 1/5, 1/10, and 1/20 w/w) are evaluated. All the systems and their multiscale organization are studied using different experimental techniques, including rheology, circular dichroism, Fourier transform infrared spectroscopy, and scanning electron microscopy (SEM). Preliminary profiles of biocompatibility for the studied systems are also described by testing them in vitro on HaCaT and 3T3-L1 cell lines. Additionally, the lysine (K) residue at the C-terminus of the Fmoc-FF moiety introduces into the supramolecular material chemical functions (amino groups) which may be useful for modification/derivatization with bioactive molecules of interest, including diagnostic probes, chelating agents, active pharmaceutical ingredients, or peptide nucleic acids.