硒(Se)维持根际土壤-植物稳态,并调节信号分子以减轻镉(Cd)毒性。然而,关于纳米硒(nano-Se)对Cd胁迫下辣椒植物非目标代谢产物和营养成分调节的影响,尚无系统的研究。本研究研究了Cd污染土壤胁迫和纳米Se(1、5和20mg/L)对代谢机制的影响。水果营养品质,和辣椒植物的挥发性有机化合物(VOCs)组成。根和果实中差异代谢产物的筛选表明,大部分参与氨基酸代谢和辣椒素的产生。根中的氨基酸(Pro,Trp,Arg,和Gln)和水果(Phe,Glu,Pro,Arg,Trp,和Gln)通过纳米硒生物强化显着提高。苯丙烷分支脂肪酸途径基因的表达(BCAT,胖,AT3,HCT,和Kas)由纳米硒(5毫克/升)诱导,增加辣椒素的水平(29.6%),去甲二氢辣椒素(44.2%),和二氢辣椒素(45.3%)。VOCs(戊醇,芳樟醇氧化物,E-2-庚醛,2-己烯醛,巴豆酸乙酯,与作物抗性和品质相关的2-丁酮)与纳米Se浓度相对应而显着增加。因此,纳米硒可以通过调节辣椒素代谢途径和调节氨基酸和VOC含量来改善辣椒植物的健康。
Selenium (Se) maintains soil-plant homeostasis in the rhizosphere and regulates signaling molecules to mitigate cadmium (Cd) toxicity. However, there has been no systematic investigation of the effects of nano-selenium (nano-Se) on the regulation of non-target metabolites and nutritional components in pepper plants under Cd stress. This study investigated the effects of Cd-contaminated soil stress and nano-Se (1, 5, and 20 mg/L) on the metabolic mechanism, fruit nutritional quality, and volatile organic compounds (VOCs) composition of pepper plants. The screening of differential metabolites in roots and fruit showed that most were involved in amino acid metabolism and capsaicin production. Amino acids in roots (Pro, Trp, Arg, and Gln) and fruits (Phe, Glu, Pro, Arg, Trp, and Gln) were dramatically elevated by nano-Se biofortification. The expression of genes of the phenylpropane-branched fatty acid pathway (BCAT, Fat, AT3, HCT, and Kas) was induced by nano-Se (5 mg/L), increasing the levels of capsaicin (29.6%), nordihydrocapsaicin (44.2%), and dihydrocapsaicin (45.3%). VOCs (amyl alcohol, linalool oxide, E-2-heptaldehyde, 2-hexenal, ethyl crotonate, and 2-butanone) related to crop resistance and quality were markedly increased in correspondence with the nano-Se concentration. Therefore, nano-Se can improve the health of pepper plants by regulating the capsaicin metabolic pathway and modulating both amino acid and VOC contents.