本研究调查了A.mellifera肠道微生物群多样性和酶活性,旨在将已确定的分离株用于可持续作物残留管理和土壤健康增强的实际应用。这项研究对蜜蜂进行了采样,通过16SrRNA基因分析肠道细菌多样性,并筛选分离物的纤维素分解,半纤维素分解,和果胶分解活性,随后评估酶的潜力。该研究表明,纤维素分解和半纤维素分解细菌分离,主要来自γ-变形杆菌,放线菌,和Firmicutes,对作物残留管理具有巨大潜力。一些属,像动脉瘤杆菌,芽孢杆菌,梭菌属,肠杆菌,Serratia,窄食单胞菌,Apilactacillus,溶血芽孢杆菌,和假单胞菌,非常擅长分解纤维素和半纤维素酶。著名的纤维素降解属包括雪松(1.390±0.57),梭菌属(1.360±0.86U/mg),肠杆菌(1.493±1.10U/mg),克雷伯菌(1.380±2.03U/mg),沙雷症(1.402±0.31U/mg),而动脉瘤(1.213±1.12U/mg),芽孢杆菌(3.119±0.55U/mg),肠杆菌(1.042±0.14U/mg),沙雷症(1.589±0.05U/mg),黄单胞菌(1.156±0.08U/mg)的半纤维素酶活性优异。鉴定了具有高纤维素分解和半纤维素分解活性的特定分离株,强调他们在作物残留管理方面的潜力。该研究探索了A.mellifera的肠道细菌区室化,强调肠道生理学在纤维素和半纤维素消化中的作用。观察到果胶分解活性,特别是在芽孢杆菌科进化枝(3.229±0.02)中,有助于了解蜜蜂肠道微生物组。这些发现提供了对微生物组多样性和酶能力的见解,对生物技术在可持续作物残留管理中的应用具有重要意义。该研究最后强调需要进行持续的研究,以揭示影响肠道微生物群的潜在机制和生态因素。影响蜜蜂的健康,菌落动力学,以及作物残留管理的进步。
This study investigates A. mellifera gut microbiota diversity and enzymatic activities, aiming to utilize identified isolates for practical applications in sustainable crop residue management and soil health enhancement. This study sampled honey bees, analyzed gut bacterial diversity via 16S rRNA gene, and screened isolates for cellulolytic, hemicellulolytic, and pectinolytic activities, with subsequent assessment of enzymatic potential. The study reveals that cellulolytic and hemicellulolytic bacterial isolates, mainly from γ-Proteobacteria, Actinobacteria, and Firmicutes, have significant potential for crop residue management. Some genera, like Aneurinibacillus, Bacillus, Clostridium, Enterobacter, Serratia, Stenotrophomonas, Apilactobacillus, Lysinibacillus, and Pseudomonas, are very good at breaking down cellulose and hemicellulase. Notable cellulose-degrading genera include Cedecea (1.390 ± 0.57), Clostridium (1.360 ± 0.86 U/mg), Enterobacter (1.493 ± 1.10 U/mg), Klebsiella (1.380 ± 2.03 U/mg), and Serratia (1.402 ± 0.31 U/mg), while Aneurinibacillus (1.213 ± 1.12 U/mg), Bacillus (3.119 ± 0.55 U/mg), Enterobacter (1.042 ± 0.14 U/mg), Serratia (1.589 ± 0.05 U/mg), and Xanthomonas (1.156 ± 0.08 U/mg) excel in hemicellulase activity. Specific isolates with high cellulolytic and hemicellulolytic activities are identified, highlighting their potential for crop residue management. The research explores gut bacterial compartmentalization in A. mellifera, emphasising gut physiology\'s role in cellulose and hemicellulose digestion. Pectinolytic activity is observed, particularly in the Bacillaceae clade (3.229 ± 0.02), contributing to understanding the honey bee gut microbiome. The findings offer insights into microbiome diversity and enzymatic capabilities, with implications for biotechnological applications in sustainable crop residue management. The study concludes by emphasizing the need for ongoing research to uncover underlying mechanisms and ecological factors influencing gut microbiota, impacting honey bee health, colony dynamics, and advancements in crop residue management.