节肢动物在全球范围内存在广泛且不断扩大,可以传播各种病毒,细菌,和寄生虫病原体。与蜱有关的许多立克次体和东方物种,跳蚤,虱子,和螨虫已经被检测到,或与世隔绝,世界各地有发热性疾病和/或动物水库的患者。蚊子目前不被认为是立克次体的媒介。对人类或动物的病原体。在这项研究中,我们对475个伊蚊池进行了随机宏基因组下一代测序(NGS),Culex,2018年至2019年在格鲁吉亚收集的库利西塔蚊子种类,在33只蚊子中鉴定了立克次体基因序列。我们通过属特异性定量PCR(qPCR)和多位点序列分型(MLST)进一步证实了立克次体的发现。NGS和MLST结果表明立克次体属。与贝利立克次体密切相关,这对人类来说是未知的。结果,以及立克次体属的其他报道。在蚊子和易感性和传播性实验中,表明蚊子可能在立克次体的传播周期中起作用。
Arthropods have a broad and expanding worldwide presence and can transmit a variety of viral, bacterial, and parasite pathogens. A number of Rickettsia and Orientia species associated with ticks, fleas, lice, and mites have been detected in, or isolated from, patients with febrile illness and/or animal reservoirs throughout the world. Mosquitoes are not currently considered vectors for Rickettsia spp. pathogens to humans or to animals. In this study, we conducted a random metagenome next-generation sequencing (NGS) of 475 pools of Aedes, Culex, and Culiseta species of mosquitoes collected in Georgia from 2018 to 2019, identifying rickettsial gene sequences in 33 pools of mosquitoes. We further confirmed the findings of the Rickettsia by genus-specific quantitative PCR (qPCR) and multi-locus sequence typing (MLST). The NGS and MLST results indicate that Rickettsia spp. are closely related to Rickettsia bellii, which is not known to be pathogenic in humans. The results, together with other reports of Rickettsia spp. in mosquitoes and the susceptibility and transmissibility experiments, suggest that mosquitoes may play a role in the transmission cycle of Rickettsia spp.