背景:由于其蛋白质含量高,因此在无谷物的狗饮食中,脉冲成分通常会代替谷物。然而,确定这种修改的好处的研究是有限的。
目的:将一种含玉米的对照饮食(Ctl)和三种无谷物饮食中的食物化合物与增加的全豆类内含物(高达45%;Pulse15,Pulse30,Pulse45)相关联,在20周内,健康犬的餐后氨基酸(AA)可以满足类似的宏量和微量营养素目标。
方法:使用串联MS分析饮食中的生化化合物。二十八个户外住宅,健康,成年西伯利亚爱斯基摩犬在基线和第2,4,8,16和20周进行饮食和膳食反应,在禁食和用餐后15,30,60,90,120和180分钟收集样本.通过UPLC分析血液AAs,并在周之间进行差异分析,在SASStudio中分析治疗和餐后时间。在SASStudio中使用饮食中的生化化合物作为预测变量并使用血液AA作为响应变量进行偏最小二乘回归。
结果:在血浆中,与Pulse15相比,Pulse45的餐后Asn高出32%,Ctl的餐后Leu高出34%,与Pulse15相比Pro高出35%(P<0.05)。在全血中,Pulse30的餐后Lys比Ctl高23%,与Pulse45和Pulse30相比,Ctl餐后Met高21%,Pro高18%(P<0.05)。几种磷脂与餐后AAs相关。“尿素循环”和“甘氨酸和丝氨酸代谢”中的化合物在血浆和全血中更富集(P<0.05),分别。
结论:在大量和微量营养素平衡的犬饮食中,它们包含玉米和豆类来源的成分,餐后循环AA的变化在很大程度上表明了饮食AA。这有助于我们进一步了解饲喂无谷物饮食的健康狗的AA代谢。
BACKGROUND: Pulse ingredients often replace grains in grain-free dog diets owing to their high-protein content. However, research to ascertain the benefit of this modification is limited.
OBJECTIVE: This study aimed to correlate food compounds in 1 corn-inclusive control diet and 3 grain-free diets with increasing inclusions of whole pulses (≤45%; Pulse15, Pulse30, and Pulse45), formulated to meet similar macronutrient and micronutrient targets with postprandial amino acids (AAs) in healthy dogs >20 wk.
METHODS: Diets were analyzed for biochemical compounds using tandem mass spectrometry. Twenty-eight outdoor-housed, healthy, adult Siberian Huskies were allocated to diet, and meal responses were analyzed at baseline and weeks 2, 4, 8, 16, and 20 with samples collected at fasted and 15, 30, 60, 90, 120, and 180 min after meal presentation. Blood AAs were analyzed by ultra performance liquid chromatography and differences across week, treatment, and time postmeal were analyzed in SAS Studio. Partial least squares regression was performed in SAS Studio using biochemical compounds in the diet as predictor variables and blood AAs as response variables.
RESULTS: In plasma, Pulse45 had ∼32% greater postprandial Asn than Pulse15, and the control diet had ∼34% greater postprandial Leu and ∼35% greater Pro than Pulse15 (P < 0.05). In whole blood, Pulse30 had ∼23% greater postprandial Lys than the control diet, and the control diet had ∼21% greater postprandial Met and ∼18% greater Pro than Pulse45 and Pulse30, respectively (P < 0.05). Several phospholipids were correlated with postprandial AAs. Compounds in the urea cycle and glycine and serine metabolism were more enriched (P < 0.05) in plasma and whole blood, respectively.
CONCLUSIONS: In macronutrient-balanced and micronutrient-balanced canine diets that differ in their inclusion of corn-derived compared with pulse-derived ingredients, postprandial changes in circulating AAs are largely indicative of the dietary AAs. This helps further our understanding of AA metabolism in healthy dogs fed grain-free diets.