■环状RNA(circularRNAs)在肿瘤进展中具有重要作用。已经报道了源自ARP2肌动蛋白相关蛋白2同源物(ciractr2)的circRNA在各种人类疾病中的作用。然而,cirACTR2在膀胱癌(BCa)中的功能和调节机制尚不清楚。
■本研究旨在探讨circACTR2在BCa中的生物学作用和调节机制。
■我们通过生物信息学分析分析了ciractr2对BCa的影响,RT-qPCR,和细胞功能测定。
■我们观察到BCa组织中circACTR2的上调,并验证了其圆形结构。功能丧失试验证明沉默cirACTR2能抑制增殖,入侵,和BCa细胞的迁移。机制研究表明,circACTR2海绵miR-219a-2-3p可提高核因子κB激酶亚基β(IKBKB)抑制剂的表达。这种诱导的IKKβ蛋白上调促进了p65的核易位,从而激活了NF-κB信号通路。
■我们的发现表明circACTR2促进BCa细胞增殖,迁移,通过miR-219a-2-3p/IKBKB轴激活NF-κB信号通路,可能揭示BCa的新治疗靶点。
UNASSIGNED: Circular RNAs (circRNAs) have significant roles in tumor progression. The role of circRNA derived from ARP2 actin-related protein 2 homolog (circACTR2) has been reported in various human diseases. However, the functions and regulatory mechanisms of circACTR2 in Bladder Cancer (BCa) remain unknown.
UNASSIGNED: This study aims to explore the biological role and regulatory mechanism of circACTR2 in BCa.
UNASSIGNED: We analyzed the effects of circACTR2 on BCa through bioinformatics analyses, RT-qPCR, and cell function assays.
UNASSIGNED: We observed the upregulation of circACTR2 in BCa tissues and validated its circular structure. Loss-of-function assays demonstrated that silencing circACTR2 suppressed the proliferation, invasion, and migration of BCa cells. Mechanistic investigation revealed that circACTR2 sponges miR-219a-2-3p to elevate the expression of the inhibitor of nuclear factor kappa B kinase subunit beta (IKBKB). This induced upregulation of IKKβ protein promoted the nuclear translocation of
p65, thereby activating the NF-κB signaling pathway.
UNASSIGNED: Our findings indicate that circACTR2 promotes BCa cell proliferation, migration, and invasion by activating the NF-κB signaling pathway via the miR-219a-2-3p/IKBKB axis, potentially unveiling a new therapeutic target for BCa.