关节软骨缺损是一个全球性的挑战,造成严重残疾。修复大型缺陷是有问题的,经常超过软骨的自愈能力和破坏骨结构。为了解决这个问题,我们开发了一种支架介导的治疗性离子递送系统。这些支架由聚(ε-己内酯)和锶(Sr)掺杂的生物活性纳米玻璃(SrBGn)构成,创建一个独特的分层结构,具有3D打印的大孔,微孔,和纳米拓扑由于SrBGn集成。SrBGn嵌入支架(SrBGn-μCh)释放Sr,硅(Si),和钙(Ca)离子,改善软骨细胞活化,附着力,扩散,和成熟相关的基因表达。这种多离子递送显著影响软骨细胞的代谢活性和成熟。重要的是,Sr离子可能通过Notch信号通路参与软骨细胞的调节。值得注意的是,脚手架的结构和拓扑线索加速了招募,附着力,传播,软骨细胞和骨髓间充质干细胞的增殖。Si和Ca离子加速成骨分化和血管形成,而Sr离子增强M2巨噬细胞的极化。我们的发现表明,SrBGn-μCh支架通过传递多种离子并提供结构/拓扑线索来加速骨软骨缺损的修复,最终支持宿主细胞功能和缺损愈合。这种支架对于骨软骨修复应用具有很大的前景。本文受版权保护。保留所有权利。
Articular cartilage defects are a global challenge, causing substantial disability. Repairing large defects is problematic, often exceeding cartilage\'s self-healing capacity and damaging bone structures. To tackle this problem, a scaffold-mediated therapeutic ion delivery system is developed. These scaffolds are constructed from poly(ε-caprolactone) and strontium (Sr)-doped bioactive nanoglasses (SrBGn), creating a unique hierarchical structure featuring macropores from 3D printing, micropores, and nanotopologies due to SrBGn integration. The SrBGn-embedded scaffolds (SrBGn-µCh) release Sr, silicon (Si), and calcium (Ca) ions, which improve chondrocyte activation, adhesion, proliferation, and maturation-related gene expression. This multiple ion delivery significantly affects metabolic activity and maturation of chondrocytes. Importantly, Sr ions may play a role in chondrocyte regulation through the Notch signaling pathway. Notably, the scaffold\'s structure and topological cues expedite the recruitment, adhesion, spreading, and proliferation of chondrocytes and bone marrow-derived mesenchymal stem cells. Si and Ca ions accelerate osteogenic differentiation and blood vessel formation, while Sr ions enhance the polarization of M2 macrophages. The findings show that SrBGn-µCh scaffolds accelerate osteochondral defect repair by delivering multiple ions and providing structural/topological cues, ultimately supporting host cell functions and defect healing. This scaffold holds great promise for osteochondral repair applications.