本研究旨在开发银纳米粒子(AgNP)掺杂的Ti6Al4V合金表面,并研究其对代表性的外周病原体的抗菌性能和对成骨细胞的潜在细胞毒性。
通过电化学沉积将不同尺寸分布(5和30nm)的AgNPs掺入到Ti6Al4V表面上,使用具有增加的AgNP浓度(100、200和300ppm)的胶体银分散体。通过原子吸收光谱法(AAS)评估从样品表面到细胞培养基的银的时程释放。细胞附件,通过SEM研究活力和增殖,活/死染色MTT和BrdU测定。通过连续稀释斑点测定来评估针对牙龈卟啉单胞菌和中间卟啉单胞菌的抗菌作用。
观察到从实验表面释放的依赖于时间和浓度的银。总的来说,AgNP掺杂表面上的细胞活力和附着,表明在所有浓度下都有足够的细胞相容性。对于5nm大小的组,在24小时检测到短暂的细胞毒性作用,在稍后的时间点完全恢复。而对于30nm大小的组没有观察到细胞毒性。具有统计学意义的,所有AgNP组在48h时诱导细胞增殖速率的浓度依赖性降低,然后在涂覆有5nm大小的AgNP的组中在72小时恢复。具有统计学意义的,对两种围病原体均证实了高达30%的浓度依赖性抗菌作用。
这项研究揭示了AgNP掺杂的Ti6Al4V表面的最佳尺寸相关浓度,以实现抗菌效果,没有随后的细胞毒性。这些结果显着有助于开发用于口腔种植的抗菌表面。
This study aimed to develop silver nanoparticle (AgNP)-doped Ti6Al4V alloy surfaces and investigate their antibacterial properties against representative periopathogens and potential cytotoxicity on osteoblastic cells.
AgNPs of different size distributions (5 and 30nm) were incorporated onto the Ti6Al4V surfaces by electrochemical deposition, using colloid silver dispersions with increasing AgNP concentrations (100, 200 and 300ppm). The time-course silver release from the specimen surfaces to cell culture media was assessed by Atomic Absorption Spectroscopy (AAS). Cell attachment, viability and proliferation were investigated by SEM, live/dead staining MTT and BrdU assays. The antibacterial effects were assessed against P. gingivalis and P. intermedia by serial dilution spotting assays.
A time- and concentration-dependent silver release from the experimental surfaces was observed. Overall, cell viability and attachment on the AgNP-doped surfaces, suggested adequate cytocompatibility at all concentrations. A transient cytotoxic effect was detected at 24h for the 5nm-sized groups that fully recovered at later time-points, while no cytotoxicity was observed for the 30nm-sized groups. A statistically significant, concentration-dependent decrease in cell proliferation rates was induced at 48h in all AgNP groups, followed by recovery at 72h in the groups coated with 5nm-sized AgNPs. A statistically significant, concentration-dependent antibacterial effect up to 30% was confirmed against both periopathogens.
This study sheds light to the optimal size-related concentrations of AgNP-doped Ti6Al4V surfaces to achieve antibacterial effects, without subsequent cytotoxicity. These results significantly contribute to the development of antibacterial surfaces for application in oral implantology.