扇贝多刺龙虾(Panulirushomarus)养殖是解决龙虾供求矛盾的首选策略。环境条件,比如盐度,是龙虾水产养殖成功的关键。然而,还没有很好地研究了阳草对盐度胁迫的生理反应。这项研究调查了ill的组织学,在盐度28(对照)下培养的早期幼年P.homarus(体重19.04±3.95g)的渗透调节和g转录组,18,和38为6周。结果表明,在低盐度条件下,由于水的吸收和肿胀,使血吸虫的鳞片和上皮细胞严重分离,以及角质层的溶解和变薄以及分隔传入和传出通道的隔膜的破裂。与外部培养基盐度成比例地变化,并且始终高于环境渗透压。血清Na+,Cl-,K+,和Mg2+浓度的假单胞菌表现出与血清渗透压相似的模式,而Ca2浓度在盐度18时不受影响,但在盐度38时显着增加。在两种盐分胁迫下,血吸虫的GillNa+/K+-ATPase活性均增加(p<0.05)。盐度18显着增加了人马肝胰腺中的谷氨酸脱氢酶(GDH)和谷丙转氨酶(GPT)活性(p<0.05)。根据转录组分析,与对照组(盐度28)相比,分别在盐度18和38时,在P.horarus的the中获得了929和1095个差异表达基因(DEGs),这些DEGs主要参与能量代谢,跨膜运输和氧化应激和物质代谢。此外,8个关键DEGs的表达模式主要与氨基酸代谢有关,通过定量实时PCR(RT-qPCR)验证跨膜转运和氧化应激。本研究表明,盐度18比盐度38对P.homarus具有更大的影响,并且P.homarus通过生理和功能适应表现出有效的渗透调节和处理盐度波动(18至38)。本研究提供了一个更好的理解的生理应答策略的P。这对于优化该物种的水产养殖实践至关重要。
Scalloped spiny lobster (Panulirus homarus) aquaculture is the preferred strategy to resolve the conflict between supply and demand for lobster. Environmental conditions, such as salinity, are key to the success of lobster aquaculture. However, physiological responses of P. homarus to salinity stress have not been well studied. This study investigated the gill histology,
osmoregulation and gill transcriptome of the early juvenile P. homarus (weight 19.04 ± 3.95 g) cultured at salinity 28 (control), 18, and 38 for 6 weeks. The results showed that the gill filaments of P. homarus exposed to low salinity showed severe separation of the cuticle and epithelial cells due to water absorption and swelling, as well as the dissolution and thinning of the cuticle and the rupture of the septum that separates the afferent and efferent channels. The serum osmolarity of P. homarus varied proportionately with external medium salinity and remained consistently above ambient osmolarity. The serum Na+, Cl-, K+, and Mg2+ concentrations P. homarus exhibited a pattern similar to that of serum osmolality, while the concentration of Ca2+ remained unaffected at salinity 18 but significantly increased at salinity 38. Gill Na+/K+-ATPase activity of P. homarus increased (p < 0.05) under the both salinity stress. Salinity 18 significantly increased Glutamate dehydrogenase (GDH) and Glutamicpyruvic transaminase (GPT) activity in the hepatopancreas of P. homarus (p < 0.05). According to transcriptome analysis, versus control group (salinity 28), 929 and 1095 differentially expressed genes (DEGs) were obtained in the gills of P. homarus at salinity 18 and 38, respectively, with these DEGs were mainly involved in energy metabolism, transmembrane transport and oxidative stress and substance metabolism. In addition, the expression patterns of 8 key DEGs mainly related to amino acid metabolism, transmembrane transport and oxidative stress were verified by quantitative real-time PCR (RT-qPCR). The present study suggests that salinity 18 has a greater impact on P. homarus than salinity 38, and P. homarus demonstrates effective
osmoregulation and handle with salinity fluctuations (18 to 38) through physiological and functional adaptations. This study provides an improved understanding of the physiological response strategies of P. homarus facing salinity stress, which is crucial for optimizing aquaculture practices for this species.