目的:老年受试者补充维生素D对各种健康相关参数有积极影响。我们在雄激素更年期大鼠模型中应用维生素D3后,对肾上腺皮质进行了有关维生素D活性和皮质酮输出因素的功能组织学分析。
方法:中年Wistar大鼠分为假手术(SO;n=8),兰花切除(Orx;n=8)和维生素D3处理的兰花切除(Orxvit。D;n=8)组。维生素D3(5μg/kgb.m.)皮下给药三周,而SO和Orx组单独接收了车辆。使用组织化学/免疫组织化学实现了设定的目标,体视学,超微结构和生化分析。
结果:睾丸切除术(Orx)降低了肾上腺皮质相关血管的体积密度(p<0.0001),维生素D受体(VDR;p<0.0166),细胞色素P450氧化酶2R1(CYP2R1;p<0,0001)和细胞色素P450氧化酶24(CYP24;p<0,0001)库,但增加了细胞色素P45027B1(CYP27B1;p<0,0001)库的体积密度。在Orx+vit中。D老鼠,肾上腺皮质相关胶原体积密度的增加(p<0.0001),观察到肾上腺皮质外部束状带细胞中的VDR(p<0,0001)和CYP2R1(p<0,0001)库以及脂滴直径(p<0,0001),而血管的体积密度降低(p<0.0001),CYP27B1(p<0,0001)和CYP24(p<0,0001)仓库已注册,所有人对Orx组。ACTH的血浆水平降低(p=0.0155),25-羟基维生素D3和皮质酮的血清浓度升高(分别为p<0.0001和p=0.0187),经过同样的治疗。
结论:将维生素D3应用于更年期大鼠后,皮质酮的输出增加似乎与25-羟基维生素D3的可用性增加和1,25-二羟基维生素D3在肾上腺组织中的降解减少无关,而是涉及中央监管机制。
OBJECTIVE: Vitamin D supplementation in aging subjects manifests a positive effect on various health-related parameters. We performed a functionally-histological analysis of the adrenal cortex regarding the factors of vitamin D activity and corticosterone output after vitamin D3 application in a rat model of the andropause.
METHODS: Middle-aged Wistar rats were divided into sham operated (SO; n=8), orchidectomized (Orx; n=8) and vitamin D3-treated orchidectomized (Orx+vit. D; n=8) groups. Vitamin D3 (5 μg/kg b.m.) was administered subcutaneously for three weeks, while the SO and Orx groups received the vehicle alone. Set objectives were achieved using histochemistry/immunohistochemistry, stereology, ultrastructural and biochemical analyses.
RESULTS: Orchidectomy (Orx) decreased the adrenal cortex-related volume densities of vascular (p<0,0001), vitamin D receptor (VDR; p<0,0166), cytochrome P450 oxidase 2R1 (CYP 2R1; p<0,0001) and cytochrome P450 oxidase 24 (CYP 24; p<0,0001) depots, but increased the volume density of cytochrome P450 27B1 (CYP 27B1; p<0,0001) depots. In Orx+vit. D rats, increase of the adrenal cortex-related volume densities of collagen (p<0,0001), VDR (p<0,0001) and CYP 2R1 (p<0,0001) depots as well as the lipid-droplet diameter (p<0,0001) in adrenocortical outer zona fasciculata cells was observed, while a decrease of volume densities of the vascular (p<0,0001), CYP 27B1 (p<0,0001) and CYP 24 (p<0,0001) depots was registered, all versus Orx group. Plasma level of ACTH was decreased (p=0,0155) and serum concentrations of 25-hydroxyvitamin D3 and corticosterone were increased (p<0,0001 and p=0,0187, respectively), all after the same treatment.
CONCLUSIONS: Increased corticosterone output after vitamin D3 application to andropausal rats appears not to be related to increased availability of 25-hydroxyvitamin D3 and decreased degradation of 1,25-dihydroxyvitamin D3 in adrenal tissue, but rather involves the central regulatory mechanisms.