目的:比较源自玻璃化卵母细胞的人类胚胎和源自新鲜收集卵母细胞的胚胎在卵母细胞捐献周期中的形态动力学模式。
方法:一项回顾性观察性研究设置:胚胎移植生育诊所,胚胎学实验室,塞萨洛尼基,希腊患者(S):该研究包括58个卵母细胞捐赠周期的421个玻璃化卵母细胞和23个卵母细胞捐赠周期的196个新鲜卵母细胞的胚胎。
方法:无主要结果度量(S):关键时间参数,动态事件,受精率,变性率,卵裂率,囊胚率,怀孕率,临床妊娠率,着床率和活产率。
结果:玻璃化卵母细胞的存活率为92.58%(±7.42)。两组受精率差异显著(VITRI组:71.92%±20.29,对照组:80.65%±15.22,p=0.045),乳沟,胚泡,怀孕,临床妊娠,正在怀孕,来自新鲜或玻璃化卵母细胞的胚胎之间的着床率和活产率没有显着差异。延时分析显示,任何关键时间参数均无显着差异。然而,检查动态参数时,CC1[t2-tPB2:从第二极体挤出(tPB2)到2个细胞(t2)]显示出显着差异(p=0.004),而CC1a[t2-tPNf:从原核褪色(tPNf)到2个细胞(t2)]处于显着性阈值(p=0.057)。
结论:与新鲜卵母细胞相比,玻璃化卵母细胞中的CC1表现出相对较慢的进展。相反,与新鲜卵母细胞相比,玻璃化卵母细胞中的CC1a表现出更快的进展。值得注意的是,这些临时偏差对后续发展影响很小。尽管玻璃化组的临床结果显示下降,均无统计学意义。这种意义的缺乏可以归因于有限的研究规模。
OBJECTIVE: To compare the morphokinetic patterns of human embryos originating from vitrified oocytes (VITRI group) with those derived from freshly collected oocytes (CONTROL group) in oocyte donation cycles.
METHODS: This is a retrospective observational study.
METHODS: Embryolab Fertility Clinic, Embryology Lab, Thessaloniki, Greece.
METHODS: The study included embryos from 421 vitrified oocytes from 58 oocyte donation cycles and 196 fresh oocytes from 23 oocyte donation cycles.
METHODS: None.
METHODS: Key time parameters, dynamic events, fertilization rates, degeneration rates, cleavage rates, blastocyst rates, pregnancy rates, clinical pregnancy rates, implantation rates, and live birth rates were estimated.
RESULTS: The mean survival rate of vitrified oocytes was 92.58% (±7.42%). Fertilization rates were significantly different between the 2 groups (VITRI group: 71.92% ± 20.29% and CONTROL group: 80.65% ± 15.22%) whereas the degeneration, cleavage, blastocyst, pregnancy, clinical pregnancy, ongoing pregnancy, implantation, and live birth rates were not significantly different between embryos derived from fresh or vitrified oocytes. Time-lapse analysis showed no significant difference in any key time parameter. However, when examining dynamic parameters, first cell cycle (CC1) (t2 - tPB2: from the second polar body extrusion (tPB2) up to 2 cells (t2)) showed a significant difference whereas CC1a (t2 - tPNf: from fading of the pronuclei (tPNf) up to 2 cells (t2)) was at the threshold of significance.
CONCLUSIONS: CC1 in vitrified oocytes exhibited a comparatively slower progression in contrast to fresh oocytes. Conversely, CC1a in vitrified oocytes demonstrated faster progression compared with fresh oocytes. It is worth noting that these temporary deviations had minimal impact on the subsequent development. Despite the clinical outcomes showing a decrease in the vitrified group, none of them reached statistical significance. This lack of significance could be attributed to the limited sample size of the study.