已知吸气肌肉训练(IMT)在耐力体育活动中促进生理益处并改善身体表现。然而,不同IMT处方策略促进的代谢适应仍不清楚.在这项工作中,纵向,随机化,双盲,假控制,我们进行了平行试验,以研究在不同运动强度下进行11周(3天·week-1)IMT对血清代谢组学谱及其主要调节代谢途径的影响.28名健康男性休闲自行车手(30.4±6.5岁)随机分为三组:假(6cm·H2O的吸气压力,n=7),中等强度(MI组,60%最大吸气压力(MIP),n=11)和高强度(HI组,85-90%MIP,n=10)。在IMT进行11周之前和之后收集血清样品,并通过1HNMR和UHPLC-HRMS/MS进行分析。使用线性混合模型和代谢物集富集分析来分析数据。1HNMR和UHPLC-HRMS/MS技术得到46和200个化合物,分别。这些结果表明,酮体代谢,脂肪酸生物合成,氨基酰基-tRNA生物合成在IMT后上调,而α亚麻酸和亚油酸的代谢以及不饱和脂肪酸的生物合成均下调。MI组提出了更高的MIP,色氨酸,和缬氨酸水平,但与其他两个研究组相比,2-羟基丁酸酯水平降低。这些结果表明,在不同强度的IMT后,氧化代谢过程会增加,并且有更多证据表明MI组中必需氨基酸代谢的上调伴随着呼吸肌强度的更大改善。
Inspiratory muscle training (IMT) is known to promote physiological benefits and improve physical performance in endurance sports activities. However, the metabolic adaptations promoted by different IMT prescribing strategies remain unclear. In this work, a longitudinal, randomized, double-blind, sham-controlled, parallel trial was performed to investigate the effects of 11 weeks (3 days·week-1) of IMT at different exercise intensities on the serum metabolomics profile and its main regulated metabolic pathways. Twenty-eight healthy male recreational cyclists (30.4 ± 6.5 years) were randomized into three groups: sham (6 cm·H2O of inspiratory pressure, n = 7), moderate-intensity (MI group, 60% maximal inspiratory pressure (MIP), n = 11) and high-intensity (HI group, 85-90% MIP, n = 10). Blood serum samples were collected before and after 11 weeks of IMT and analyzed by 1H NMR and UHPLC-HRMS/MS. Data were analyzed using linear mixed models and metabolite set enrichment analysis. The 1H NMR and UHPLC-HRMS/MS techniques resulted in 46 and 200 compounds, respectively. These results showed that ketone body metabolism, fatty acid biosynthesis, and aminoacyl-tRNA biosynthesis were upregulated after IMT, while alpha linolenic acid and linoleic acid metabolism as well as biosynthesis of unsaturated fatty acids were downregulated. The MI group presented higher MIP, Tryptophan, and Valine levels but decreased 2-Hydroxybutyrate levels when compared to the other two studied groups. These results suggest an increase in the oxidative metabolic processes after IMT at different intensities with additional evidence for the upregulation of essential amino acid metabolism in the MI group accompanied by greater improvement in respiratory muscle strength.