■红细胞中的低聚α-突触核蛋白(RBC-o-α-Syn)已显示在帕金森病(PD)患者中增加。然而,影响RBC-o-α-Syn水平的因素仍有待阐明。本研究的目的是分析RBC-o-α-Syn水平与年龄之间的相关性。PD患者的性别和不同的临床变量。
■本研究纳入了167名PD患者和119名健康对照(HC)。PD患者根据MDS临床PD诊断标准进行诊断。对所有参与者的临床特征进行评估。Western印迹分析用于检测RBC-o-α-Syn的分子大小。新建立的化学发光免疫测定法用于测量RBC-o-α-Syn水平。
■在PD患者中检测到比HC受试者更高的RBC-o-α-Syn水平。受试者工作特征(ROC)曲线表明,55.29ng/mg的截断值可以很好地区分PD患者和HC受试者。灵敏度为67.66%(95%CI:60.24-74.29%),特异性为88.24%(95%CI:81.22-92.86%),曲线下面积(AUC)为0.857。女性患者的RBC-o-α-Syn水平高于男性患者(p=0.033)。对于不同的亚型,MIX亚型中RBC-o-α-Syn的水平高于震颤显性(TD)PD。此外,与没有认知障碍的患者相比,RBC-o-α-Syn的水平更高(p=0.016),与简易精神状态检查(MMSE)得分呈负相关(r=-0.156,p=0.044)。
■我们的研究表明,PD患者的RBC-o-α-Syn水平高于HC受试者,并且受性别和认知障碍严重程度的影响。
UNASSIGNED: Oligomeric alpha-synuclein in red blood cells (RBC-o-α-Syn) has been shown to be increased in patients with Parkinson\'s disease (PD). However, factors that affect RBC-o-α-Syn levels remain to be elucidated. The aim of this study is to analyze the correlations between RBC-o-α-Syn levels and the age, sex and different clinical variables of patients with PD.
UNASSIGNED: 167 patients with PD and 119 healthy controls (HC) were enrolled in this study. The patients with PD were diagnosed based on the MDS clinical diagnostic criteria for PD. All participants were evaluated for their clinical characteristics. Western blot analysis was used to examine the molecular sizes of RBC-o-α-Syn. A newly established chemiluminescent immunoassay was used to measure RBC-o-α-Syn levels.
UNASSIGNED: Higher RBC-o-α-Syn levels were detected in PD patients than in HC subjects. The receiver operating characteristic (ROC) curve indicated that a cut off value of 55.29 ng/mg discriminated well between PD patients and HC subjects, with a sensitivity of 67.66% (95% CI: 60.24-74.29%), a specificity of 88.24% (95% CI: 81.22-92.86%), and an area under the curve (AUC) of 0.857. The levels of RBC-o-α-Syn were higher in female than male patients (p = 0.033). For different subtypes, the levels of RBC-o-α-Syn were higher in the MIX subtype than the tremor-dominant (TD) PD. In addition, the levels of RBC-o-α-Syn were higher in patients with than without cognitive impairment (p = 0.016), and negatively correlated with Mini-Mental State Examination (MMSE) scores (r = -0.156, p = 0.044).
UNASSIGNED: Our study demonstrates that RBC-o-α-Syn levels in patients with PD are higher than those in HC subjects and affected by the sex and the severity of cognitive impairment.