Microalgae have emerged as a promising source of biomass to produce renewable biofuels due to their ability to synthesize high-energy density compounds of commercial interest. This study proposes an approach for pilot-scale oil extraction, purification by fractional distillation, hydrocarbon characterization by gas chromatography coupled to mass spectrometry (GC-MS), evaluation of physicochemical parameters of the produced hydrocarbons, preliminary cost analysis, and challenges and future opportunities for green diesel on a commercial scale. Here, the microalgae Tetradesmus obliquus was cultivated in 12 m³ photobioreactors using biodigested swine waste as a culture medium. The resulting biomass was subjected to drying and harvesting, followed by oil extraction using a hot solvent extraction method, followed by distillation to purify the compounds. Three different extraction and distillation experiments were conducted, each using different solvent combinations. The results obtained revealed that extraction with a solvent blend, composed of hexane and ethanol, provided more significant yields compared to extraction with pure hexane. GC-MS analysis showed the presence of alkanes and alkenes in the oil samples, and the proportion of solvent used in the extraction directly influenced the production of alkanes. Additionally, specific hydrocarbons such as 4-methyl-1-decene, 8-heptadecene, 1-pentadecene, 9-heneicosene, and 2-dodecene were identified. The evaluation of the physicochemical parameters demonstrated that the calorific value of the distilled oil samples is within the range of typical values for petroleum diesel. However, it was observed that the distilled oil samples had higher sulfur content compared to conventional diesel. Regarding the cost analysis, it was found that it varies depending on the experimental conditions. In particular, the process using a solvent mixture of 70% hexane and 30% ethanol proved to be more economical than the others, since it extracted a greater quantity of oil with a lower initial biomass requirement. In summary, this microalgae-derived hydrocarbon production process is promising and offers insights for compound purification and future biofuel applications.

This research analyses 24 years of oil extraction in blocks 16 and 67 of the Yasuní National Park (YNP) in the Amazonian Forest of Ecuador, one of the most biodiverse spaces in the world and with the current presence of ancient indigenous communities. As a novel contribution, we have carried out a Life-Cycle Assessment (LCA) that quantifies the footprints associated with the extraction, transportation, refining, distribution and final uses of the oil in four different scenarios (oil for asphalt use, electricity, marine fuel and passenger car transport). This study also sheds light on the energy return at the point of use of different oil-derivatives, and complements this with a qualitative analysis of the social, cultural and environmental implications for the Waorani communities. We conclude that the environmental burdens of the extraction process in blocks 16 and 67 in 2015 were greater than those of countries such as the United States, Saudi Arabia and Indonesia, based on the analysis of 11 impact categories. The blocks\' operation is the most unfavourable for the categories of Terrestrial Acidification Potential (TAP), Global Warming Potential (GWP), Terrestrial Ecotoxicity Potential (TEP) and Ecosystem Quality Loss Potential (EQL), with increments of 804.15 %, 105.36 %, 506.29 % and 210.73 %, respectively, in relation to the average of the rest of the extraction systems analysed. Specifically, the present case study shows 75.18 % higher impacts in the blocks addressed, when compared to the Ecuadorian average. During the period 1999-2022, the carbon emissions associated with the oil extraction in these blocks have increased by 139.01%. It has been detected a neo-colonial economic behaviour: while the Ecuadorian state received 21% of the sales, the Spanish government and the oil companies received, on average, 38% and 41% of the per-litre average fuel price, respectively. Thus, 79% of the income stayed in the Global North. We conclude that, on average, 19.64 % of the impacts associated with crude oil production and consumption occur in the Amazonian region of the YNP, depending on the fuel used and the consumption mechanism. For the Global Warming Potential (GWP) impact category, the extraction process carries, on average, 34.51 % of the weight in all of the life-cycle impacts, depending on the consumption scenario. It was also estimated that to be able to use 0.33 kWh of electricity from fuel combustion, 0.47 kWh of energy for goods transport and 0.20 kWh for passenger transport, an investment of 1 kWh is required, with an average extended EROI of 1:3.33. According to the qualitative analysis performed, it has been concluded that the main local impacts are related to the obstacles in environmental monitoring and information, the economic dependence of the communities on the oil extraction company, and cultural transformations; impacts that are not easily quantifiable or detectable using other methodologies. The combination of the qualitative analysis and LCA showed that the neo-colonial economic distribution did not compensate the social and environmental impacts of the oil extraction occurred in the YNP.

Background: The growing popularity of nutrient-rich foods, among which is quinoa, is due to the increasing demand for healthier choices. Oils and hydrolyzed proteins from these foods may help prevent various health issues. The objective of this work was to perform extraction from the endosperm of the grain from high-protein quinoa flour by physical means via a differential abrasive milling process and extracting the oil using an automatic auger extractor at 160°C, as well as characterizing extracted oil. Methods: Quinoa oil extraction and physicochemical characterization were carried out. Chemical and physical quality indexes of quinoa oil were established, and both characterizations were conducted based on international and Columbian standards. Thermal properties were evaluated by differential scanning calorimetry, and rheological and interfacial properties of the oil were evaluated using hybrid rheometers and Drop Tensiometers, respectively, to determine its potential for obtaining functional foods. Results: The result was 10.5 g of oil/ 100 g of endosperm, with a moisture content of 0.12%, insoluble impurities of 0.017%, peroxide index of 18.5 meq O 2/kg of oil, saponification index of 189.6 mg potassium hydroxide/g of oil, refractive index of 1.401, and a density of 0.9179 g/cm 3 at 20°C. Regarding contaminating metals, it presented 7 mg of iron/kg of oil, a value higher than previously established limits of 5 mg of iron/kg of oil. The oil contained 24.9% oleic acid, 55.3% linoleic acid, and 4% linolenic acid, demonstrating antioxidant capacity. Quinoa oil showed thermal properties similar to other commercial oils. Conclusions: The interfacial and rheological properties were suitable for the stabilization of emulsions, gels, and foams, which are important in various industrial applications and could facilitate the development of new products. The extracted quinoa oil presented similar characteristics to other commercial oils, which could make it a potential product for commercialization and application in different industries.

In droplet microfluidics, UV-Vis absorption spectroscopy along with colorimetric assays have been widely used for chemical and biochemical analysis. However, the sensitivity of the measurement can be limited by the short optical pathlength. Here we report a novel design to enhance the sensitivity by removing oil and converting the droplets into a single-phase aqueous flow, which can be measured within a U-shape channel with long optical pathlength. The flow cells were fabricated via 3D printing. The calibration results have demonstrated complete oil removal and effective optical pathlengths similar to the designed channel lengths (from 5 to 20 mm). The flow cell was further employed in a droplet microfluidic-based phosphate sensing system. The measured phosphate levels displayed excellent consistency with data obtained from traditional UV spectroscopy analysis. This flow cell design overcomes the limitations of short optical pathlengths in droplet microfluidics and has the potential to be used for in situ and continuous monitoring.

Sesame oil has been widely used in the daily diet due to its high nutritional value. Sesame oil is extracted at industrial scales and also in small scale by cold pressing at different temperatures. In this research, sesame oil was extracted by pressing at four temperatures, namely, 30 (control sample), 60, 90 and 120 °C, to evaluate its effects on the quality of extracted oils. Oil extraction yields were increased from 38 to 51% by increasing the pressing temperature. The highest amount of peroxide and acid values were related to the oil extracted at 120 °C. Tocopherols and total phenol content were reduced by the increasing the pressing temperature, and the highest amounts of these bioactive components were related to the control sample. The results of the fatty acids profile showed that the composition of oils extracted at different temperatures did not differ significantly (p > 0.05). The results of the present study give a clear picture about the effects of different pressing temperatures on the sesame oil quality and extraction yield, and can be useful in the extraction unit optimization.

This study optimized the input processing factors, namely compression force, pressing speed, heating temperature, and heating time, for extracting oil from desiccated coconut medium using a vertical compression process by applying a maximum load of 100 kN. The samples\' pressing height of 100 mm was measured using a vessel chamber of diameter 60 mm with a plunger. The Box-Behnken design was used to generate the factors\' combinations of 27 experimental runs with each input factor set at three levels. The response surface regression technique was used to determine the optimum input factors of the calculated responses: oil yield (%), oil expression efficiency (%), and energy (J). The optimum factors\' levels were the compression force 65 kN, pressing speed 5 mm min-1, heating temperature 80 °C, and heating time 52.5 min. The predicted values of the responses were 48.48%, 78.35%, and 749.58 J. These values were validated based on additional experiments producing 48.18 ± 0.45%, 77.86 ± 0.72%, and 731.36 ± 8.04 J. The percentage error values between the experimental and the predicted values ranged from 0.82 ± 0.65 to 2.43 ± 1.07%, confirming the suitability of the established regression models for estimating the responses.

In this study, oil was extracted from black cumin (Nigella sativa) seed (BCS) by press, and oil was extracted from the obtained cake with a solvent. The changes in the quality of both crude oils obtained by pressing and by solvent were investigated during refining. Findings revealed that the p-anisidin value (p-AV) and fatty acid profile did not change significantly, but there were significant differences (p < .05) in the peroxide value (PV), reflective index, pigment contents, free fatty acid content (FFA%), and antioxidant activity (total phenol content (TPC), thymoquinone, and DPPH inhibition) of BCS oils obtained by the two different methods. PV and FFA decreased to less than 15 meqO2/kg and 0.3%, respectively, in the refined oil. The TPC (65%), thymoquinone (45-97%), carotenoids (86-89%), and chlorophyll (75-85%) were removed from BCS oil, but the DPPH value was raised by about 33%. The current study gives a clear picture of the changes during refining in BCS oil, which can be a useful guide in food applications.

Oil has been extracted from the Western Amazon since the 1920s, leading to severe environmental contamination due to frequent occurrence oil spills and the dumping of produced water. Local inhabitants, along with environmental and human rights organizations, have reported the adverse effects of oil-related pollution on their livelihoods and the ecosystems they depend on. Here, we study accumulation of oil-related heavy metals in wildlife, and its subsequent incorporation into the trophic chain. We analysed the concentration of 14 heavy metals (Cd, Cr, Hg, As, Ni, V, Ba, Se, Be, Fe, Cu, Zn, Mn, Al) in liver samples from 78 lowland pacas (Cuniculus paca) hunted for subsistence in an oil-polluted area from the northern Peruvian Amazon where oil has been extracted since the 1970s (n = 38), and two control areas, the Yavari-Mirín River basin (n = 20), and the Pucacuro River basin (n = 20). Pacas in the oil-polluted area have significantly higher concentrations of Cd (P < 0.01) and Ba (P < 0.0001) compared to those in control areas, suggesting bioaccumulation of oil-related pollution. Conversely, Se levels were significantly lower in the oil-polluted area (P < 0.0001), likely due to the sequestration of Se by other heavy metals, particularly Cd. Additionally, minor variations in other heavy metals, e.g., Fe and Zn, were observed in pacas from the oil-polluted area, whereas control areas showed higher concentrations of Ni and Cu. Mn and Al levels did not significantly differ between the study areas. These results underscore the impact of oil extraction on the absorption and assimilation of heavy metals in wildlife, point at oil activities as the source of the high and unsafe blood Cd levels reported for the indigenous population of the studied oil extraction area and raise concerns about the long-term health risks from oil extraction posed to local Indigenous People who rely on subsistence hunting.

This study aimed to characterize acorn oil (AO) and carnauba wax-based acorn oil oleogel (AOG) and the effect of AOG replacement on the textural and sensorial properties of chocolate spread. Oil yields from cold-pressing (Quercus longipes) were around 14%wt with a nice nutty smell. The main fatty acids of AO were included oleic, linoleic, and palmitic acid (44, 38, and 10%wt) respectively. The prepared AOG using 6%wt of carnauba wax (CW) showed high strength (G\' > 100 mPa) and oil binding capacity ∼87 %. Based on microstructure assays platelet-like and β\' polymorphic triglyceride crystalline networks were formed in AOG. The Pickering AOG/water emulsions in the volumetric ratio of from 90:10 up to 40:60 were stable due to the placement of CW-based AOG particles at the interface of water/oil as Pickering stabilizer. The high physical stability of the emulgel against phase separation is considered an important advantage for using oleogel in chocolate spread formulations instead of vegetable oils, which usually have a high percentage of oil release. The spreads prepared by replacing 50%wt AOG with butter showed acceptable textural and sensorial properties.

Background: The valorization of byproducts to obtain high nutritional value foods is of utmost importance for our planet where the population is booming. Among these products are oils rich in ω-3 fatty acids produced from fishery byproducts. Recently, mullet roe oil from roe byproducts was produced that was rich in the ω-3 fatty acids eicosatetraenoic acid (EPA) and docosahexaenoic acid (DHA). Oils are customarily characterized for their composition and degree of oxidation but little is known of their biological effects, especially the effect of the extraction method. Methods: The purpose of this study was to evaluate the effects of freshly extracted mullet roe oil from mullet roe byproducts and the effect of the extraction method on human red blood cells (hRBCs) and platelets. To this end, the hemocompatibility (cytotoxicity), oxidative effects, and erythrocyte membrane changes were examined after 1 and 24 h of incubation. Antiplatelet effects were also assessed in vitro. Results: The expeller press oil extraction method and alcalase-assisted extraction produced the most biocompatible oils, as shown by hemocompatibility measurements and the absence of erythrocyte membrane alterations. Solvent extracts and protease-assisted extraction oils resulted in the rupture of red blood cells at different examined dilutions, creating hemolysis. Conclusions: It seems that the proper functioning of oil-erythrocyte interactions cannot be explained solely by ROS. Further investigations combining chemical analysis with oil-cell interactions could be used as an input to design high nutritional value oils using green extraction technologies. All samples exhibited promising antiplatelet and antiblood clotting effects in vitro.