■肠道屏障,包括肠道微生物群,在慢性肾脏病(CKD)的进展和营养状况中起着关键作用。本研究旨在探讨血液透析(HD)患者的肠道屏障改变,非HD(NHD)CKD患者,健康的志愿者
■我们的横断面研究纳入了22名HD患者,11名NHD患者,11名健康志愿者我们评估了粪便微生物群组成(通过细菌16SrRNA基因测序评估),粪便IgA水平,肠道通透性的替代标记,血清细胞因子,食欲调节者,营养状况,身体活动,和生活质量。
■与健康志愿者相比,HD患者的粪便微生物组成发生了显着变化,观察到类群的变化,已知与饮食模式或产生作用于人类宿主的代谢物有关。与健康志愿者相比,HD患者的个体表现出炎症标志物水平升高(CRP,IL-6和TNF-α),胰高血糖素样肽-2和潜在的食欲减退标志物(包括瘦素和肽YY)。NHD患者CRP和肽YY水平升高。整体粪便微生物群组成与身高有关,软瘦质量,静息能量消耗,握力,骨矿物质含量和血浆白蛋白和TNF-α。
■与健康志愿者相比,HD患者的粪便微生物组成改变,更高的全身性炎症,以及食欲调节因子血浆水平的改变。虽然有些差异与以前的发现一致,异质性的存在可能是由于各种因素,包括生活方式和合并症。尽管有样本量等限制,我们的研究强调了肠道微生物群之间多方面的相互作用,生理标记,和肾功能,保证在更大的队列中进行进一步的调查。
UNASSIGNED: The gut barrier, comprising gut microbiota, plays a pivotal role in chronic kidney disease (CKD) progression and nutritional status. This study aimed to explore gut barrier alterations in hemodialyzed (HD) patients, non-HD (NHD) CKD patients, and healthy volunteers.
UNASSIGNED: Our cross-sectional study enrolled 22 HD patients, 11 NHD patients, and 11 healthy volunteers. We evaluated fecal microbiota composition (assessed via bacterial 16S rRNA gene sequencing), fecal IgA levels, surrogate markers of gut permeability, serum cytokines, appetite mediators, nutritional status, physical activity, and quality of life.
UNASSIGNED: HD patients exhibited significant alterations in fecal microbiota composition compared to healthy volunteers, with observed shifts in taxa known to be associated with dietary patterns or producing metabolites acting on human host. In comparison to healthy volunteers, individuals with HD patients exhibited elevated levels of inflammatory markers (CRP, IL-6 and TNF-α), glucagon-like peptide-2, and potential anorexigenic markers (including leptin and peptide YY). NHD patients had increased levels of CRP and peptide YY. Overall fecal microbiota composition was associated with height, soft lean mass, resting energy expenditure, handgrip strength, bone mineral content and plasma albumin and TNF-α.
UNASSIGNED: Compared to healthy volunteers, HD patients have an altered fecal microbiota composition, a higher systemic inflammation, and a modification in plasma levels of appetite mediators. While some differences align with previous findings, heterogeneity exists likely due to various factors including lifestyle and comorbidities. Despite limitations such as sample size, our study underscores the multifaceted interplay between gut microbiota, physiological markers, and kidney function, warranting further investigation in larger cohorts.