未经授权:卵巢雌二醇支持女性性行为和代谢功能。虽然啮齿动物的卵巢切除术(OVX)消除了性行为并导致肥胖,非人灵长类动物的OVX减少,但没有废除,性行为,并且不一致地改变体重增加。
UNASSIGNED:我们假设卵巢外雌二醇为这两种功能提供了关键支持,为了测试这个想法,我们通过抑制芳香化酶来消除卵巢外雌二醇生物合成和饮食诱导的肥胖,从而促进体重增加.
UNASSIGNED:13名成年雌性mar猴接受了OVX,并接受了(1)含雌二醇的胶囊和每日口服治疗的媒介物(E2;n=5);空胶囊和每日口服治疗(2)媒介物(VEH,1mL/kg,n=4),或(3)来曲唑(LET,1mg/kg,n=4)。
未经批准:7个月后,我们观察到E2的强烈性接受性,VEH的中频,而在LET女性中几乎没有(P=.04)。相比之下,在E2中观察到很少的男性坐骑排斥,在VEH中观察到中频,以及LET女性的高频率(P=.04)。在E2中始终观察到接受性头部转动,但在VEH和LET女性中未观察到。让女性,独自一人,对男性表现出强烈的侵略性排斥。VEH和LET女性表现出增加的体重增加%(P=.01)。外周血中雌二醇的相对水平为E2>>>VEH>LET,而那些在下丘脑排名E2=VEH>LET,证实来曲唑对下丘脑雌二醇合成的抑制作用。
未经调查:我们的发现为非人类灵长类动物卵巢外雌二醇有助于女性性行为提供了第一个证据,并迅速推测卵巢外雌二醇,特别是神经雌激素,可以类似地调节其他灵长类动物的性动机,包括人类。
UNASSIGNED: Ovarian estradiol supports female sexual behavior and metabolic function. While ovariectomy (OVX) in rodents abolishes sexual behavior and enables obesity, OVX in nonhuman primates decreases, but does not abolish, sexual behavior, and inconsistently alters weight gain.
UNASSIGNED: We hypothesize that extra-ovarian estradiol provides key support for both functions, and to test this idea, we employed aromatase inhibition to eliminate extra-ovarian estradiol biosynthesis and diet-induced obesity to enhance weight gain.
UNASSIGNED: Thirteen adult female marmosets were OVX and received (1) estradiol-containing capsules and daily oral treatments of vehicle (E2; n = 5); empty capsules and daily oral treatments of either (2) vehicle (VEH, 1 mL/kg, n = 4), or (3) letrozole (LET, 1 mg/kg, n = 4).
UNASSIGNED: After 7 months, we observed robust sexual receptivity in E2, intermediate frequencies in VEH, and virtually none in LET females (P = .04). By contrast, few rejections of male mounts were observed in E2, intermediate frequencies in VEH, and high frequencies in LET females (P = .04). Receptive head turns were consistently observed in E2, but not in VEH and LET females. LET females, alone, exhibited robust aggressive rejection of males. VEH and LET females demonstrated increased % body weight gain (P = .01). Relative estradiol levels in peripheral serum were E2 >>> VEH > LET, while those in hypothalamus ranked E2 = VEH > LET, confirming inhibition of local hypothalamic estradiol synthesis by letrozole.
UNASSIGNED: Our findings provide the first evidence for extra-ovarian estradiol contributing to female sexual behavior in a nonhuman primate, and prompt speculation that extra-ovarian estradiol, and in particular neuroestrogens, may similarly regulate sexual motivation in other primates, including humans.