目的:环境因素如噪音和音乐可以显著影响生理反应,包括炎症。这项研究探讨了环境因素,如噪音和音乐如何影响脂多糖(LPS)诱导的炎症,专注于系统和器官特异性反应。
方法:24只Wistar大鼠分为4组(每组6只):对照组,LPS组,噪声暴露组,和音乐曝光组。所有的老鼠,除了控制组,腹腔注射10mg/kgLPS。噪声暴露组大鼠暴露于95分贝噪声,和音乐曝光组听莫扎特的K.448音乐(65-75分贝)每天1小时超过7天。酶联免疫吸附试验用于检测炎症细胞因子的水平,包括肿瘤坏死因子-α(TNF-α)和白细胞介素-1β(IL-1β),在血清和组织(肺,肝脏,和肾脏)。Westernblot检测了器官组织中核因子-κB(NF-κB)p65的磷酸化水平。
结果:与对照组相比,LPS诱导的脓毒症大鼠血清中TNF-α和IL-1β水平显著升高,肺,肝脏,和肾脏组织,以及在肺中p-NF-κBp65蛋白表达显著升高,肝脏,和肾脏组织。噪声暴露进一步放大了这些炎症标志物,而音乐暴露在LPS诱导的脓毒症大鼠中减少了它们。
结论:噪声暴露通过激活NF-κB途径加剧炎症,导致脓毒症期间炎症标志物的上调。相反,音乐暴露抑制NF-κB信号,表明在减少炎症的潜在治疗效果。
OBJECTIVE: Environmental factors such as
noise and music can significantly impact physiological responses, including inflammation. This study explored how environmental factors like
noise and music affect lipopolysaccharide (LPS)-induced inflammation, with a focus on systemic and organ-specific responses.
METHODS: 24 Wistar rats were divided into four groups (n = 6 per group): Control group, LPS group, noise-exposed group, and music-exposed group. All rats, except for the Control group, received 10 mg/kg LPS intraperitoneally. The rats in the
noise-exposed group were exposed to 95 dB
noise, and the music-exposed group listened to Mozart\'s K. 448 music (65-75 dB) for 1 h daily over 7 days. An enzyme-linked immunosorbent assay was utilized to detect the levels of inflammatory cytokines, including tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β), in serum and tissues (lung, liver, and kidney). Western blot examined the phosphorylation levels of nuclear factor-κB (NF-κB) p65 in organ tissues.
RESULTS: Compared with the Control group, LPS-induced sepsis rats displayed a significant increase in the levels of TNF-α and IL-1β in serum, lung, liver, and kidney tissues, as well as a remarkable elevation in the p-NF-κB p65 protein expression in lung, liver, and kidney tissues.
Noise exposure further amplified these inflammatory markers, while music exposure reduced them in LPS-induced sepsis rats.
CONCLUSIONS: Noise exposure exacerbates inflammation by activating the NF-κB pathway, leading to the up-regulation of inflammatory markers during sepsis. On the contrary, music exposure inhibits NF-κB signaling, indicating a potential therapeutic effect in reducing inflammation.