背景:β-烟酰胺单核苷酸是“抗衰老”的重要突破,并在数量上始终处于最畅销的营养补充剂列表中。作为β-烟酰胺单核苷酸的代谢产物,烟酰胺和N1-甲基烟酰胺的检测对于评价膳食补充剂β-烟酰胺单核苷酸的营养效果具有重要意义。然而,由于体内烟酰胺和N1-甲基烟酰胺的浓度极低,生物样品中存在严重的基质干扰,对材料和预处理方法的需求日益增加。
结果:在这项研究中,首次合成了Fe3O4@羟丙基甲基纤维素@十二烷基苯磺酸磁流体,并将其作为前处理材料,采用高效液相色谱法检测尿液样品中的烟酰胺和N1-甲基烟酰胺。与其他吸附材料相比,Fe3O4@羟丙基甲基纤维素@十二烷基苯磺酸纳米粒子是一种均匀的磁流体。此外,它们有更多类型和数量的相互作用位点(静电相互作用,疏水相互作用,氢键相互作用,和π-π相互作用),所以他们有更大的吸附能力。当溶液的pH为4时,它们可以在10s内快速吸附。该方法成功地在0.1-80μgmL-1的线性范围内检测到尿液样品中的痕量烟酰胺和N1-甲基烟酰胺,检测下限分别为0.30ngmL-1和1.5ngmL-1,定量限分别为1.0ngmL-1和5.0ngmL-1。同时,烟酰胺和N1-甲基烟酰胺的标准尿液样品显示令人满意的回收率94.50-109.1%。结果表明,该方法能准确、定量测定尿样中的烟酰胺和N1-甲基烟酰胺。
结论:因此,低浓度的β-烟酰胺单核苷酸代谢物可以同时检测,在检测过程中可以消除干扰,为β-烟酰胺单核苷酸代谢物的分析提供了一种高效、便捷的前处理方法和快速、灵敏的检测方法。更重要的是,在生物样品中其他类似代谢物的分析中具有广泛的应用前景。
BACKGROUND: β-nicotinamide mononucleotide stands out as an essential breakthrough in \"anti-aging\" and consistently leads the list of top-selling nutritional supplements in terms of quantity. As the metabolites of β-nicotinamide mononucleotide, the detection of nicotinamide and N1-methylnicotinamide is of great significance for evaluating the nutritional effect of dietary supplements of β-nicotinamide mononucleotide. However, due to the extremely low concentration of nicotinamide and N1-methylnicotinamide in vivo and the serious matrix interference in biological samples, there is an increasing demand for materials and methods of pre-treatment.
RESULTS: In this study, Fe3O4@hydroxypropyl methyl cellulose@dodecylbenzenesulfonic acid magnetic fluid was synthesized for the first time, and it was used as pretreatment material to detect nicotinamide and N1- methylnicotinamide in urine samples by high performance liquid chromatography. Compared with other adsorption materials, Fe3O4@hydroxypropyl methyl cellulose@dodecylbenzenesulfonic acid nanoparticles are a kind of uniform magnetic fluid. Furthermore, they have more types and quantities of interaction sites (electrostatic interactions, hydrophobic interactions, hydrogen bonding interactions, and π-π interactions), so they own greater adsorption capacity. When the pH of the solution is 4, they can be adsorbed quickly within 10 s. The method successfully detected trace nicotinamide and N1-methylnicotinamide in urine samples in the linear range of 0.1-80 μg mL-1, the low detection limits were 0.30 ng mL-1 and 1.5 ng mL-1 respectively, and the quantification limits were 1.0 ng mL-1 and 5.0 ng mL-1, respectively. At the same time, the standard urine samples of nicotinamide and N1-methylnicotinamide showed satisfactory recovery rate 94.50-109.1 %. The results indicated that the established method can accurately and quantitatively determine trace nicotinamide and N1-methylnicotinamide in urine samples.
CONCLUSIONS: Consequently, low concentration of β-nicotinamide mononucleotide metabolites can be detected simultaneously, and the interference can be eliminated during the detection process, which provides an efficient and convenient pretreatment method and a rapid and sensitive detection method for the analysis of β-nicotinamide mononucleotide metabolites. What\'s more, it has a wide application prospect in the analysis of other similar metabolites in biological samples.