阳离子表面活性剂用于许多工业过程和消费品中,同时释放到水生环境中。它们可能在水生生物中积累到相关的阈值。这里,我们旨在更好地了解三种选定的阳离子表面活性剂的生物富集行为,即N,N-二甲基癸胺(T10),N-甲基十二烷基胺(S12),N,N,N-三甲基十四烷基铵阳离子(Q14),在鱼肝(RTL-W1)和g(RTgill-W1)细胞系的细胞中。我们用细胞培养物进行了生物浓缩试验的完全质量平衡,在这种情况下,细胞表面,细胞本身,对塑料隔室进行取样,并通过HPLCMS/MS对每种表面活性剂进行定量。与表面活性剂烷基链长度及其膜脂质-水分配系数相关的细胞中/细胞的积累,DMLW.T10和S12的细胞衍生的生物富集因子(BCF)在从文献获得的体内BCF的3.5倍内,而Q14的细胞来源的BCF值高于体内BCF>100倍。从我们的实验来看,虹鳟鱼细胞系似乎是一种合适的保守的体外筛选方法,用于阳离子表面活性剂的生物浓缩评估,并有望用于进一步的测试。
Cationic surfactants are used in many industrial processes and in consumer products with concurrent release into the aquatic environment, where they may accumulate in aquatic organisms to regulatoryly relevant thresholds. Here, we aimed to better understand the bioconcentration behavior of three selected cationic surfactants, namely N,N-dimethyldecylamine (T10), N-methyldodecylamine (S12), and N,N,N-trimethyltetradecylammonium cation (Q14), in the cells of fish liver (RTL-W1) and gill (RTgill-W1) cell lines. We conducted full mass balances for bioconcentration tests with the cell cultures, in which the medium, the cell surface, the cells themselves, and the plastic compartment were sampled and quantified for each surfactant by HPLC MS/MS. Accumulation in/to cells correlated with the surfactants\' alkyl chain lengths and their membrane lipid-water partitioning coefficient, DMLW. Cell-derived bioconcentration factors (BCF) of T10 and S12 were within a factor of 3.5 to in vivo BCF obtained from the literature, while the cell-derived BCF values for Q14 were >100 times higher than the in vivo BCF. From our experiments, rainbow trout cell lines appear as a suitable conservative in vitro screening method for bioconcentration assessment of cationic surfactants and are promising for further testing.