背景:刺激,一种能够生产和储存花蜜的结构,不仅在授粉过程中起着至关重要的作用,而且还促进了一些植物谱系的快速多样化,这被认为是工厂的关键创新。骨刺是许多研究的焦点,如进化和生态假说,但是目前对马刺发展的理解是有限的。对凤仙花进行了高通量测序,研究了其骨刺发育的分子机制,这被认为可以为凤仙花的发展提供一些见解。
结果:在三个发育阶段对I.uliginosa的骨刺和四肢进行了转录测序和分析。总共获得了47.83Gb的干净数据,组装了49,716个基因。与NR比较后,Swiss-Prot,普法姆,COG,GO和KEGG数据库,共有27686个基因被成功注释。通过比较分析,发现了19,356个差异表达基因,并富集到208个GO术语和146个KEGG途径中,其中植物激素信号转导是最显著的富集途径。鉴定了一千三十二个转录因子,属于33个TF家族,如MYB,bHLH和TCP。通过qPCR筛选并验证了20个可能参与骨刺发育的候选基因。例如SBP,IAA和ABP。
结论:获得了马刺不同发育阶段的转录组数据,并鉴定了一系列与骨刺发育相关的候选基因。与细胞周期相关的基因的重要性,细胞分裂,阐明了骨刺发育中的细胞伸长和激素。本研究为了解凤仙花骨刺发育的分子机制提供了有价值的信息和资源。
BACKGROUND: Spur, a structure capable of producing and storing nectar, not only plays a vital role in the pollination process but also promotes the rapid diversification of some plant lineages, which is considered a key innovation in plants. Spur is the focus of many studies, such as evolution and ecological hypothesis, but the current understanding of spur development is limited. High-throughput sequencing of Impatiens uliginosa was carried out to study the molecular mechanism of its spur development, which is believed to provide some insights into the spur development of Impatiens.
RESULTS: Transcriptomic sequencing and analysis were performed on spurs and limbs of I. uliginosa at three developmental stages. A total of 47.83 Gb of clean data were obtained, and 49,716 unigene genes were assembled. After comparison with NR, Swiss-Prot, Pfam, COG, GO and KEGG databases, a total of 27,686 genes were annotated successfully. Through comparative analysis, 19,356 differentially expressed genes were found and enriched into 208 GO terms and 146 KEGG pathways, among which plant hormone signal transduction was the most significantly enriched pathway. One thousand thirty-two transcription factors were identified, which belonged to 33 TF families such as MYB, bHLH and TCP. Twenty candidate genes that may be involved in spur development were screened and verified by qPCR, such as SBP, IAA and ABP.
CONCLUSIONS: Transcriptome data of different developmental stages of spurs were obtained, and a series of candidate genes related to spur development were identified. The importance of genes related to cell cycle, cell division, cell elongation and hormones in spur development was clarified. This study provided valuable information and resources for understanding the molecular mechanism of spur development in Impatiens.