■外周血干细胞(PBSC)捐赠是用于收集造血干细胞移植的造血干细胞和祖细胞(HSPC)的主要程序。单次运动可短暂富集外周血HSPCs和溶细胞性自然杀伤细胞(CD56dim),这对预防移植后并发症很重要。为研究在PBSC捐赠环境中锻炼的效用(≈3小时)提供一个理论基础,本研究旨在确定间歇循环是否比连续循环增加外周血HSPC和CD56dim浓度。
■在随机交叉研究设计中,11名男性(平均±SD:年龄25±7岁)进行了中等强度的连续运动[MICE,30分钟,65%-70%最大心率(HRmax)],高容量高强度间歇运动(HV-IIIE,4×4分钟,80%-85%HRmax)和低容量IIIE(LV-IIIE,4×2分钟,90%-95%HRmax)。每个间隔对循环HSPC(CD34+CD45dimSSClow)和CD56dim浓度(细胞/微升)的累积影响,并确定HSPCs的骨髓归巢潜力(CXCR-4和VLA-4的表达)。
■两次LV-HIIE后HSPC浓度增加(休息:1.84±1.55vs.间隔2:2.94±1.34,P=0.01)和仅HV-HIIE的三个间隔(休息:2.05±0.86vs.区间3:2.51±1.05,P=0.04)。每次试验后,所有白细胞亚群的浓度均增加,CD56dimNK细胞最大,在IIIEvs.MICE(LV-HIIE:4.77±2.82,HV-HIIE:4.65±2.06,MICE:2.44±0.77,P<0.0001)。观察到这些模式的浓度,不是CXCR-4+和VLA-4+HSPC的频率,这是不变的。在所有试验之后,在运动动员的HSPCs上,VLA-4的表达略有下降,但CXCR-4的表达没有下降(P<0.0001)。
■本研究的结果表明,与MICE相比,HIIE引起的HSPC和CD56dimNK细胞浓度增加更为明显,动员的HSPC维持其骨髓归巢表型。LV-HIIE仅在2×2分钟间隔后引起HSPC浓度增加。因此,应评估PBSC捐赠背景下间歇循环的可行性和临床实用性。
UNASSIGNED: Peripheral blood stem cell (PBSC) donation is the primary procedure used to collect hematopoietic stem and progenitor cells (HSPCs) for hematopoietic stem cell transplantation. Single bouts of exercise transiently enrich peripheral blood with HSPCs and cytolytic natural killer cells (CD56dim), which are important in preventing post-transplant complications. To provide a rationale to investigate the utility of exercise in a PBSC donation setting (≈3 h), this study aimed to establish whether interval cycling increased peripheral blood HSPC and CD56dim concentrations to a greater degree than continuous cycling.
UNASSIGNED: In a randomised crossover study design, eleven males (mean ± SD: age 25 ± 7 years) undertook bouts of moderate intensity continuous exercise [MICE, 30 min, 65%-70% maximum heart rate (HRmax)], high-volume high intensity interval exercise (HV-HIIE, 4 × 4 min, 80%-85% HRmax) and low-volume HIIE (LV-HIIE, 4 × 2 min, 90%-95% HRmax). The cumulative impact of each interval on circulating HSPC (CD34+CD45dimSSClow) and CD56dim concentrations (cells/µL), and the bone marrow homing potential of HSPCs (expression of CXCR-4 and VLA-4) were determined.
UNASSIGNED: There was an increase in HSPC concentration after two intervals of LV-HIIE (Rest: 1.84 ± 1.55 vs. Interval 2: 2.94 ± 1.34, P = 0.01) and three intervals of HV-HIIE only (Rest: 2.05 ± 0.86 vs. Interval 3: 2.51 ± 1.05, P = 0.04). The concentration of all leukocyte subsets increased after each trial, with this greatest for CD56dim NK cells, and in HIIE vs. MICE (LV-HIIE: 4.77 ± 2.82, HV-HIIE: 4.65 ± 2.06, MICE: 2.44 ± 0.77, P < 0.0001). These patterns were observed for concentration, not frequency of CXCR-4+ and VLA-4+ HSPCs, which was unaltered. There was a marginal decrease in VLA-4, but not CXCR-4 expression on exercise-mobilised HSPCs after all trials (P < 0.0001).
UNASSIGNED: The results of the present study indicate that HIIE caused a more marked increase in HSPC and CD56dim NK cell concentrations than MICE, with mobilised HSPCs maintaining their bone marrow homing phenotype. LV-HIIE evoked an increase in HSPC concentration after just 2 × 2-minute intervals. The feasibility and clinical utility of interval cycling in a PBSC donation context should therefore be evaluated.