■在许多国家,2019年冠状病毒病(COVID-19)大流行期间,口罩已成为常见的景象。然而,长期戴口罩对健康人鼻腔微生物群的影响尚不完全清楚。
■在这项研究中,我们通过16S核糖体RNA基因测序,将82名长时间佩戴口罩的年轻成年人的鼻腔微生物群与COVID-19大流行前招募的来自同一学校的172名无口罩同龄人进行了比较.多样性,composition,并对两组鼻腔微生物群功能进行分析。通过基于培养的分析确定在鼻腔中定植的共生细菌的患病率。
■我们观察到,与2018年的无面罩对照组相比,长时间佩戴面罩的人的鼻腔微生物表征和代谢功能显着不同。具体来说,长时间戴口罩者的鼻腔微生物群显示出葡萄球菌的丰度增加,假交替单胞菌,棒状杆菌,等。同时,包括拟杆菌属在内的几个属的丰度,粪杆菌,和Agathobacter下降。此外,我们观察到COVID-19感染史对鼻腔微生物群的组成没有显著影响.此外,基于培养的分析显示金黄色葡萄球菌和痤疮棒杆菌增加,长期佩戴面罩者鼻腔表皮葡萄球菌减少。
■总的来说,我们的研究表明,长时间戴口罩可以显著改变鼻腔微生物群。
Face masks have become a common sight during the Coronavirus Disease 2019 (COVID-19) pandemic in many countries. However, the impact of prolonged face mask wearing on nasal microbiota of healthy people is not fully understood.
In this study, we compared the nasal microbiota of 82 young adults who wore face masks for an extended period of time to 172 mask-free peers from the same school recruited before the COVID-19 pandemic via 16S ribosomal RNA gene sequencing. Diversity, composition, and function of nasal microbiota between the two groups were analyzed. Prevalence of commensal bacteria colonized in the nasal cavity was determined by culture-based analysis.
We observed that prolonged face mask wearers had significantly different nasal microbial characterization and metabolic function compared to mask-free controls from 2018. Specifically, the nasal microbiota of the prolonged mask wearers displayed increased abundance of Staphylococcus, Pseudoalteromonas, Corynebacterium, etc. Meanwhile, the abundance of several genera including Bacteroides, Faecalibacterium, and Agathobacter was decreased. Moreover, we observed that COVID-19 infection history did not affect the composition of nasal microbiota significantly. Additionally, the culture-based analysis revealed that Staphylococcus aureus and Corynebacterium accolens increased, and Staphylococcus epidermidis decreased in the nasal cavity of prolonged mask wearers.
Overall, our study suggests that prolonged face mask wearing can significantly alter the nasal microbiota.