需要骨整合的骨科植入物通常经过表面修饰;然而,植入物可能会脱落这些涂层并产生磨损碎片,从而导致并发症。钛纳米管(TiNT),一种新的表面处理,可以促进骨整合。在这项研究中,体外(大鼠骨髓来源的骨髓细胞附着和形态学)和体内(髓内固定的大鼠模型)实验表征了两种TiNT表面形态的局部和全身反应,对齐和小梁,通过动物和远程器官的重量,金属离子,血液学,和非脱钙组织学分析。体外实验显示,在30分钟和4小时,小梁和对齐的TiNT表面上的总粘附细胞大于对照,和细胞的直径更小,更偏心。对照动物体重增加,然而,平均而言,没有动物符合减肥的机构触发因素.TiNT组的血液学参数(全血细胞计数与差异)没有显着差异。控制。电感耦合等离子体质谱(ICP-MS)显示,小梁TiNT组的肺中铝含量高于对照组。组织学分析显示无炎性浸润,细胞毒性,或K线附近的坏死条件。在植入小梁TiNT的股骨远端区域,嗜酸性粒细胞/嗜碱性粒细胞和嗜中性粒细胞明显减少;在对齐的TiNT植入股骨的中轴,异物巨/多核细胞和中性粒细胞明显减少,表明与对照组相比,对齐的TiNT植入股骨的免疫反应降低。
Orthopedic implants requiring osseointegration are often surface modified; however, implants may shed these coatings and generate wear debris leading to complications. Titanium nanotubes (TiNT), a new surface treatment, may promote osseointegration. In this study, in vitro (rat marrow-derived bone marrow cell attachment and morphology) and in vivo (rat model of intramedullary fixation) experiments characterized local and systemic responses of two TiNT surface morphologies, aligned and trabecular, via animal and remote organ weight, metal ion, hematologic, and nondecalcified histologic analyses. In vitro experiments showed total adherent cells on trabecular and aligned TiNT surfaces were greater than control at 30 min and 4 h, and cells were smaller in diameter and more eccentric. Control animals gained more weight, on average; however, no animals met the institutional trigger for weight loss. No hematologic parameters (complete blood count with differential) were significantly different for TiNT groups vs. control. Inductively coupled plasma mass spectrometry (ICP-MS) showed greater aluminum levels in the lungs of the trabecular TiNT group than in those of the controls. Histologic analysis demonstrated no inflammatory infiltrate, cytotoxic, or necrotic conditions in proximity of K-wires. There were significantly fewer eosinophils/basophils and neutrophils in the distal region of trabecular TiNT-implanted femora; and, in the midshaft of aligned TiNT-implanted femora, there were significantly fewer foreign body giant/multinucleated cells and neutrophils, indicating a decreased immune response in aligned TiNT-implanted femora compared to controls.