不安全的食品添加剂对全球健康构成重大威胁,尤其是在发展中国家。许多现有的方法依赖于清洁的实验室,复杂的光学设备,训练有素的人员和漫长的检测时间,不适合在紧急情况下进行现场食品安全检查,特别是在贫困地区。在本文中,通过设计基于纳米杂化(MoS2/SDBS/Cu-CuFe2O4)的催化,设计了一种快速直观的现场方法,用于检测和定量食品安全中的添加剂。有趣的是,纳米杂化物对含有3,3',5,5'-四甲基联苯胺(TMB)和过氧化氢(H2O2),然后将其简单地整合到检测试剂盒中。该试剂盒中的蓝色氧化TMB可以通过商业食品中的一些生物分子添加剂完全转化为无色,如谷胱甘肽(GSH),半胱氨酸(Cys),和抗坏血酸(AA)。值得注意的是,这个过程只需要不到2分钟,检测极限为2.8nM,5.5nM和47nM,分别。这些结果显示了超过30次测试的统计学分析(*P<0.05)的优异的可重复性。接下来,可以使用智能手机通过红-绿-蓝(RGB)通道清晰地捕获颜色变化的图像,这为现场操作装置的发展提供了契机。此外,我们的方法适用于一些目标指示性食品,回收率在95.8%至104.2%之间,为未来实用的食品安全检查应用提供有吸引力和有前途的途径。更重要的是,该方法可以很容易地推广到其他分析领域的还原性物质检测。
Unsafe food additives pose a significant threat to global health, especially in developing countries. Many existing methods rely on clean laboratories, complicated optics equipment, trained personnel and lengthy detection time, which are not suitable for onsite food safety inspections in emergency situations, peculiarly in impoverished areas. In this paper, a fast and visual onsite method is designed for the detection and quantification of additives in food safety by engineering a
nanohybrid (MoS2/SDBS/Cu-CuFe2O4)-based catalysis. Interestingly, the
nanohybrid presents peroxidase-like mimetic activity toward the substrate containing 3,3\',5,5\'-tetramethylbenzidine (TMB) and hydrogen peroxide (H2O2), which are then integrated simply into a detection kit. The blue oxidated TMB in this kit can be converted completely to colorless by some bio-molecule additives in commercial food, such as glutathione (GSH), cysteine (Cys), and ascorbic acid (AA). Remarkably, this process takes just less than 2 min and the detection limits are 2.8 nM, 5.5 nM and 47 nM, respectively. These results show excellent repeatability with a statistical analysis with (*P < 0.05) over 30 tests. Next, the images of the color changes can be captured clearly using a smartphone by red-green-blue (RGB) channels, which provides an opportunity for the development of field-operation device. Additionally, our approach is applied to some targets-indicative foods, showing a recovery range between 95.8 % and 104.2 %, offering an attractive and promising pathway for future practical food safety inspection applications. More importantly, this method can easily be extended to the detection of reducing substances in other analytical fields.