背景:伤口愈合在精细协调的炎症级联反应上,对组织修复至关重要。慢性伤口,持续的炎症和感染易感性,带来了巨大的临床挑战。纳米纤维敷料为伤口护理提供了有希望的途径,然而,它们与炎症和感染的相互作用仍然难以捉摸。我们旨在描述伤口闭合前的炎症级联反应,并评估Cu@Bbc纳米纤维在减轻炎症和抵抗感染方面的治疗功效。它们独特的属性表明在调节炎症方面有希望,促进组织再生,并防止微生物定植。研究纳米纤维支架之间复杂的相互作用,炎症,和感染可能揭示增强伤口愈合的机制。我们的发现可以刺激定制敷料的发展,在免疫失调的情况下,迫切需要有效的伤口管理,感染,和炎症。
方法:在这项调查中,我们合成了Cu@Bbc纳米纤维,掺入姜黄素和盐酸小檗碱,伤口愈合的应用。我们评估了他们的个体和联合抗菌药物,抗生物膜,和抗氧化活性,通过分子对接与促炎细胞因子的结合亲和力。通过SEM进行形态学表征,FTIR评估官能团,和润湿性接触角测量疏水性能。物理性质,包括抗拉强度,溶胀行为,和热稳定性,使用拉伸测试进行评估,盐水浸泡法和热重分析法。通过土壤掩埋试验评估纳米纤维的生物降解性。通过MTT测定生物相容性,而伤口愈合效果是通过体外划痕试验评估的。控制药物释放和抗MRSA的抗菌活性进行了检查,在斑马鱼模型中进行体内评估,阐明炎症反应和组织重塑。
结果:在这项研究中,姜黄素和盐酸小檗碱的协同作用对MRSA表现出有效的抗菌作用,具有显著的抗成熟生物膜破坏。此外,该组合显示出增强的抗氧化潜力。分子对接研究显示与促炎细胞因子的强结合亲和力,提示在加速伤口愈合至关重要的炎症反应中的作用。形态学分析证实了纳米纤维的质量,通过FTIR光谱验证药物的存在。Cu@Bbc表现出更高的拉伸强度,最佳溶胀行为,和强大的热稳定性,通过拉伸测试和热重分析评估。此外,Cu@Bbc纳米纤维显示出增强的生物降解性,正如土埋试验所证实的那样。生物相容性评估显示良好的相容性,而体外研究证明了有效的抗菌活性。体内斑马鱼实验显示伤口加速闭合,再上皮化,增强了免疫反应,表明伤口愈合增强。
结论:总之,我们的调查强调了Cu@Bbc纳米纤维的功效,载有姜黄素和盐酸小檗碱,在显示强大的抗菌和抗氧化属性,同时还调节免疫反应和炎症级联反应必不可少的伤口愈合。这些结果表明它们作为临床实施的多面伤口敷料的潜力。
BACKGROUND: Wound healing pivots on a finely orchestrated inflammatory cascade, critical for tissue repair. Chronic wounds, compounded by persistent inflammation and susceptibility to infection, pose formidable clinical challenges.
Nanofiber dressings offer promising avenues for wound care, yet their interaction with inflammation and infection remains elusive. We aim to delineate the inflammatory cascade preceding wound closure and assess Cu@Bbc nanofibers\' therapeutic efficacy in mitigating inflammation and combating infection. Their unique attributes suggest promise in modulating inflammation, fostering tissue regeneration, and preventing microbial colonization. Investigating the intricate interplay between
nanofiber scaffolds, inflammation, and infection may unveil mechanisms of enhanced wound healing. Our findings could stimulate the development of tailored dressings, urgently needed for effective wound management amidst immune dysregulation, infection, and inflammation.
METHODS: In this investigation, we synthesized Cu@Bbc nanofibers, incorporating curcumin and berberine chloride, for wound healing applications. We evaluated their individual and combined antibacterial, anti-biofilm, and antioxidant activities, alongside binding affinity with pro-inflammatory cytokines through molecular docking. Morphological characterization was conducted via SEM, FTIR assessed functional groups, and wettability contact angle measured hydrophobic properties. The physical properties, including tensile strength, swelling behavior, and thermal stability, were evaluated using tensile testing, saline immersion method and thermogravimetric analysis. Biodegradability of the nanofibers was assessed through a soil burial test. Biocompatibility was determined via MTT assay, while wound healing efficacy was assessed with in vitro scratch assays. Controlled drug release and antibacterial activity against MRSA were examined, with in vivo assessment in a zebrafish model elucidating inflammatory responses and tissue remodeling.
RESULTS: In this study, the synergistic action of curcumin and berberine chloride exhibited potent antibacterial efficacy against MRSA, with significant anti-mature biofilm disruption. Additionally, the combination demonstrated heightened antioxidant potential. Molecular docking studies revealed strong binding affinity with pro-inflammatory cytokines, suggesting a role in expediting the inflammatory response crucial for wound healing. Morphological analysis confirmed
nanofiber quality, with drug presence verified via FTIR spectroscopy. Cu@Bbc demonstrated higher tensile strength, optimal swelling behavior, and robust thermal stability as evaluated through tensile testing and thermogravimetric analysis. Additionally, the Cu@Bbc
nanofiber showed enhanced biodegradability, as confirmed by the soil burial test. Biocompatibility assessments showed favorable compatibility, while in vitro studies demonstrated potent antibacterial activity. In vivo zebrafish experiments revealed accelerated wound closure, re-epithelialization, and heightened immune response, indicative of enhanced wound healing.
CONCLUSIONS: In summary, our investigation highlights the efficacy of Cu@Bbc nanofibers, laden with curcumin and berberine chloride, in displaying robust antibacterial and antioxidant attributes while also modulating immune responses and inflammatory cascades essential for wound healing. These results signify their potential as multifaceted wound dressings for clinical implementation.