■据报道,特发性癫痫(IE)中肠道微生物组的组成和功能发生了变化,然而,在这种情况下,肠道微生物与肠神经系统(ENS)的相互作用需要进一步研究。这项初步研究检查了胃肠道微生物群(GIM),它们的代谢物,肠内容物中所含的营养物质与ENS相通。
■健康犬和IE犬的粪便上清液(FS),包括药物幼稚,苯巴比妥(PB)反应,和PB无反应的狗,将其应用于培养的肌间神经元,以使用电压敏感染料神经成像测试其激活。此外,对FS中短链脂肪酸(SCFA)的浓度进行定量。
■我们的发现表明,来自所有检查组的FS都引起神经元激活。值得注意的是,与健康对照相比,PB无反应犬的FS在较高比例的肠神经元中具有IE诱导的动作电位放电,总体上表现出最低的突发频率。此外,在暴露于患有IE的未接触药物的狗的FS时,观察到肠神经元的最高爆发频率。与在具有IE的PB无反应狗中观察到的频率相比,该频率显着更高,并且显示出超过健康对照的趋势。
■尽管在各种FS样本中观察到的SCFA浓度差异可能与诱导的神经元活动有关,在这一点上,直接的相关性仍然难以捉摸。获得的结果表明ENS参与犬IE,并为将来的研究奠定了基础。
UNASSIGNED: Alterations in the composition and function of the gut microbiome have been reported in idiopathic epilepsy (IE), however, interactions of gut microbes with the enteric nervous system (ENS) in this context require further study. This pilot study examined how gastrointestinal microbiota (GIM), their metabolites, and nutrients contained in intestinal contents communicate with the ENS.
UNASSIGNED: Fecal supernatants (FS) from healthy dogs and dogs with IE, including drug-naïve, phenobarbital (PB) responsive, and PB non-responsive dogs, were applied to cultured myenteric neurons to test their activation using voltage-sensitive dye neuroimaging. Additionally, the concentrations of short-chain fatty acids (SCFAs) in the FS were quantified.
UNASSIGNED: Our findings indicate that FS from all examined groups elicited neuronal activation. Notably, FS from PB non-responsive dogs with IE induced action potential discharge in a higher proportion of enteric neurons compared to healthy controls, which exhibited the lowest burst frequency overall. Furthermore, the highest burst frequency in enteric neurons was observed upon exposure to FS from drug-naïve dogs with IE. This frequency was significantly higher compared to that observed in PB non-responsive dogs with IE and showed a tendency to surpass that of healthy controls.
UNASSIGNED: Although observed disparities in SCFA concentrations across the various FS samples might be associated with the induced neuronal activity, a direct correlation remains elusive at this point. The obtained results hint at an involvement of the ENS in canine IE and set the basis for future studies.