麦冬(天门冬科)是多年生草种,可作为观赏植物和药用植物栽培。2021年4月至2022年9月,日照市发现了一种严重的刺槐叶枯病,山东省,中国。最初的疾病症状是小的黄色斑点,最终发展为叶枯病,通常与许多小的,黑色,在病变中形成的半浸入式比目鱼分生孢子。为了获得这种疾病的致病因子的分离株,样品是从日照市刺槐病叶中随机采集的。总共从样品中获得了97个毛竹分离株,并使用内部转录间隔区(ITS)对组合数据集的形态特征和多位点系统发育分析进行了研究,核糖体RNA的28S大亚基(LSU),和部分平移延伸因子1-α(tef),肌动蛋白(act)和甘油醛-3-磷酸脱氢酶(gapdh)基因座。系统发育,这些毛竹分离株在浓集假单胞菌物种复合体中形成了进化枝,簇生有毛孢子菌和细脊菌。形态学上,该进化枝中的分离株与毛孢子菌和尖刺菌的分生孢子细胞和分生孢子的大小不同,并且没有顶端分生孢子附属物。因此,这些分离株被描述为一种新物种。使用科赫的假设证实了致病性,这表明,在中国的日本竹节虫上可以诱发叶枯病症状。引文:王丙乙,王T-T,马C-Y,XueH,李勇,朴C-G,江宁(2023年)。毛竹。11月。在中国引起麦冬叶枯病。真菌系统学和进化11:43-50。doi:10.3114/fuse.2023.11.03。
Ophiopogon japonicus (Asparagaceae) is a perennial grass species which can be cultivated as an ornamental and medicinal plant. From April 2021 to September 2022, a serious leaf blight disease of O. japonicus was discovered in Rizhao City, Shandong Province, China. The initial disease symptoms were small yellow spots, finally developing as tip blight, often associated with many small, black, semi-immersed pycnidial conidiomata formed in lesions. To obtain isolates of the causal agent for this disease, samples were randomly collected from O. japonicus diseased leaves in Rizhao City. In total 97 Phyllosticta isolates were obtained from samples, and studied using morphological features and multi-locus phylogenetic analyses of a combined dataset using the internal transcribed spacers (ITS), the 28S large subunit of ribosomal RNA (LSU), and partial translation elongation factor 1-alpha (tef), actin (act) and glyceraldehyde-3-phosphate dehydrogenase (gapdh) loci. Phylogenetically, these Phyllosticta isolates formed a clade in the P. concentrica species complex, and clustered with P. pilospora and P. spinarum. Morphologically, isolates in this clade differed from P. pilospora and P. spinarum by the size of conidiogenous cells and conidia, and the absence of an apical conidial appendage. As a result, these isolates were described as a novel species Phyllosticta rizhaoensis. Pathogenicity was confirmed using Koch\'s postulates, which showed that P. rizhaoensis could induce leaf blight symptoms on O. japonicus in China. Citation: Wang C-B, Wang T-T, Ma C-Y, Xue H, Li Y, Piao C-G, Jiang N (2023). Phyllosticta rizhaoensis sp. nov. causing leaf blight of Ophiopogon japonicus in China. Fungal Systematics and Evolution 11: 43-50. doi: 10.3114/fuse.2023.11.03.