背景:女性生殖衰老的迅速加速已成为主要的公共卫生问题。他的羊超公式(HSYC),由八种草药组成的化合物,具有增强卵巢功能的功效。因此,需要深入研究其抗卵巢衰老机制。
目的:通过分析肠道菌群(GM),评估HSYC对自然衰老小鼠的抗卵巢衰老作用,并探讨其潜在机制。代谢组,和转录组。
方法:选择年轻和晚期母鼠(AMA)用于本研究。苏木精和伊红染色,荧光染色,西方印迹,和qPCR分析用于检测与卵巢衰老相关的表型。随后,对通用汽车的分析,转录组,进行代谢组分析以探索HSYC的潜在作用机制。最后,进行了体内和体外实验以验证潜在的治疗机制.
结果:HSYC促进AMA小鼠卵泡发育,改善与年龄相关的线粒体功能障碍,凋亡,和DNA损伤修复的缺陷。GM分析显示,HSYC处理显着增加了Akkermansia和Turicibacter的丰度。转录组和代谢组分析表明,HSYC可能通过调节代谢途径减轻卵巢衰老,氨基酸代谢,谷胱甘肽代谢,以及泛酸和辅酶A的合成。结合转录组和代谢组学分析确定谷胱甘肽代谢途径是HSYC抵抗卵巢衰老的关键途径。额外的实验验证证实,HSYC上调谷胱甘肽代谢基因GPX8,GSTA1和GSTA4,增加谷胱甘肽相关产物(GSH),并降低ROS水平。
结论:HSYC通过调节多种内源性代谢产物对卵巢衰老具有有益的治疗作用。目标,和代谢途径,强调其通过谷胱甘肽代谢途径的抗卵巢衰老作用。这些发现强调了HSYC在解决卵巢衰老方面的创新潜力,并提供了一种针对多种生物学途径的新型治疗方法,以改善AMA女性的生殖健康。.
BACKGROUND: The rapid acceleration of female reproductive aging has become a major public health concern. He\'s Yangchao formula (HSYC), a compound comprising eight herbs, has demonstrated efficacy in enhancing ovarian function. Thus, an in-depth study of its anti-ovarian aging mechanism is required.
OBJECTIVE: To evaluate the anti-ovarian aging effect of HSYC in naturally aged mice and investigate the underlying mechanism by analyzing the gut microbiota (GM), metabolome, and transcriptome.
METHODS: Young and advanced maternal age (AMA) mice were selected for this study. Hematoxylin and eosin staining, fluorescence staining, western blotting, and qPCR analyses were used to detect the phenotypes associated with ovarian aging. Subsequently, analyses of the GM, transcriptome, and metabolome analyses were performed to explore the potential mechanisms of action of HSYC. Finally, in vivo and in vitro experiments were performed to verify potential therapeutic mechanisms.
RESULTS: HSYC promoted follicular development in AMA mice and ameliorated age-related mitochondrial dysfunction, apoptosis, and defects in DNA damage repair. GM analysis revealed that HSYC treatment significantly increased the abundance of Akkermansia and Turicibacter. Transcriptome and metabolome analyses showed that HSYC might mitigate ovarian aging by regulating metabolic pathways, amino acid metabolism, glutathione metabolism, and the synthesis of pantothenic acid and coenzyme A. Combined transcriptomic and metabolomic analyses identified the glutathione metabolic pathway as the key pathway through which HSYC counteracts ovarian aging. Additional experimental verification confirmed that HSYC upregulated the glutathione metabolic genes GPX8, GSTA1, and GSTA4, increased glutathione-related products (GSH), and reduced ROS levels.
CONCLUSIONS: HSYC exerts beneficial therapeutic effects on ovarian aging by regulating multiple endogenous metabolites, targets, and metabolic pathways, with an emphasis on its anti-ovarian aging effects through the glutathione metabolic pathway. These findings underscore the innovative potential of HSYC in addressing ovarian aging and offer a novel therapeutic approach that targets multiple biological pathways to improve the reproductive health of women with AMA..