啮齿动物模型,比如老鼠和老鼠,通常用于检查眼部疾病中的视网膜神经节细胞损伤。然而,作为夜间活动的动物,啮齿动物的视网膜结构不同于灵长类动物,在研究视网膜病理学方面施加了重大限制。树sh(Tupaiabelangeri)很小,与小鼠相比,昼夜副动物表现出更高的视敏度和色觉。像人类一样,树sh具有致密的视网膜神经纤维层(RNFL)和厚的神经节细胞层(GCL),使它们成为研究视神经病变的有价值的模型。在这项研究中,我们应用高分辨率可见光光学相干层析成像技术来表征活体树sh视网膜结构,并将其与人类和小鼠的结构进行比较。我们在体内定量表征树sh的视网膜层结构,首次专门检查内部丛状层(IPL)内的子层结构。接下来,我们对树sh的视网膜层结构进行了比较分析,老鼠,和人类。然后,我们使用离体共聚焦显微镜在树the内部视网膜中验证了我们的体内发现。rew视网膜的体内和离体分析为未来的工作奠定了基础,以准确跟踪和量化IPL中的视网膜结构变化。GCL,和RNFL在人类视神经疾病的发生和发展过程中。
Rodent models, such as mice and rats, are commonly used to examine retinal ganglion cell damage in eye diseases. However, as nocturnal animals, rodent retinal structures differ from primates, imposing significant limitations in studying retinal pathology. Tree shrews (Tupaia belangeri) are small, diurnal paraprimates that exhibit superior visual acuity and color vision compared with mice. Like humans, tree shrews have a dense retinal nerve fiber layer (RNFL) and a thick ganglion cell layer (GCL), making them a valuable model for investigating optic neuropathies. In this study, we applied high-resolution visible-light optical coherence tomography to characterize the tree shrew retinal structure in vivo and compare it with that of humans and mice. We quantitatively characterize the tree shrew\'s retinal layer structure in vivo, specifically examining the sublayer structures within the inner plexiform layer (IPL) for the first time. Next, we conducted a comparative analysis of retinal layer structures among tree shrews, mice, and humans. We then validated our in vivo findings in the tree shrew inner retina using ex vivo confocal microscopy. The in vivo and ex vivo analyses of the shrew retina build the foundation for future work to accurately track and quantify the retinal structural changes in the IPL, GCL, and RNFL during the development and progression of human optic diseases.