毛竹(毛竹),以其快速增长而闻名,归因于其顶端分生组织的动态变化。已知CLAVATA3/胚胎周围区域相关(CLE)家族基因在调节模型植物的分生组织和器官形成中起关键作用。但是它们在毛竹中的功能尚不清楚。这里,我们对毛竹的CLE基因家族进行了全基因组鉴定,并研究了它们的基因结构,染色体定位,进化关系,和表达模式。共鉴定出11个PheCLE基因,所有这些都在其C末端含有保守的CLE肽核心功能基序(基序1)。根据拟南芥分类标准,这些基因主要分布在A-C组中。共线性分析揭示了毛竹CLE基因之间的显著同质性,大米,和玉米,暗示着单子叶植物进化过程中潜在的功能保守。转录组分析表明,这些基因在毛竹的顶端组织中显著表达,包括根尖,射击技巧,根茎芽,和花蕾。特别是,单细胞转录组数据和原位杂交进一步证实了PheCLE1和PheCLE10在基底根尖组织中的高表达。此外,PheCLE1和PheCLE10在水稻中的过表达显着促进了根系的生长。PheCLE1和PheCLE10均位于细胞膜上。此外,上游转录因子NAC9和NAC6表现出对PheCLE1和PheCLE10启动子的结合亲和力,从而促进其转录激活。总之,这项研究不仅首次系统地鉴定了毛竹中的CLE基因家族,而且强调了它们在根尖组织发育中的核心作用。这为进一步探索竹种中的功能肽及其信号调控网络提供了有价值的理论基础。
Moso bamboo (Phyllostachys edulis), renowned for its rapid growth, is attributed to the dynamic changes in its apical meristem. The CLAVATA3/EMBRYO SURROUNDING REGION-RELATED (CLE) family genes are known to play crucial roles in regulating meristem and organ formation in model plants, but their functions in Moso bamboo remain unclear. Here, we conducted a genome-wide identification of the CLE gene family of Moso bamboo and investigated their gene structure, chromosomal localization, evolutionary relationships, and expression patterns. A total of 11 PheCLE genes were identified, all of which contained a conserved CLE peptide core functional motif (Motif 1) at their C-termini. Based on Arabidopsis classification criteria, these genes were predominantly distributed in Groups A-C. Collinearity analysis unveiled significant synteny among CLE genes in Moso bamboo, rice, and maize, implying potential functional conservation during monocot evolution. Transcriptomic analysis showed significant expression of these genes in the apical tissues of Moso bamboo, including root tips, shoot tips, rhizome buds, and flower buds. Particularly, single-cell transcriptomic data and in situ hybridization further corroborated the heightened expression of PheCLE1 and PheCLE10 in the apical tissue of basal roots. Additionally, the overexpression of PheCLE1 and PheCLE10 in rice markedly promoted root growth. PheCLE1 and PheCLE10 were both located on the cell membrane. Furthermore, the upstream transcription factors NAC9 and NAC6 exhibited binding affinity toward the promoters of PheCLE1 and PheCLE10, thereby facilitating their transcriptional activation. In summary, this study not only systematically identified the CLE gene family in Moso bamboo for the first time but also emphasized their central roles in apical tissue development. This provides a valuable theoretical foundation for the further exploration of functional peptides and their signaling regulatory networks in bamboo species.