子实体形成是食用菌生命周期中最重要的发育事件;然而,这个过程的遗传调控还没有得到很好的理解。杏鲍菇是一种广泛栽培的蘑菇,具有很高的经济价值。两个携带相容的A和B交配型基因的单核交配是杏鲍鱼子实体发育所必需的。在这项研究中,我们表明,用相容的同源结构域(A交配型)和信息素(B交配型)基因转化的杏树单核可以完成子实体发育,但不能形成担子孢子。转录分析表明,内源性同源结构域和信息素受体基因的表达以及交配信号通路被转化子中转移的同源结构域和信息素基因激活。我们的发现为研究子实体发育提供了一个新的模型,这可能会加速未来食用蘑菇的遗传育种。重要食用蘑菇的子实体具有很高的营养价值。然而,蘑菇的子实体发育还没有得到很好的理解,因此,许多具有经济重要性的野生食用蘑菇不能人工种植。此外,可栽培蘑菇的品种略有改善。在自然条件下,子实体发育只能在核子中启动,通过交配两个相容的单核体获得的有性菌丝体。目前的工作表明,通过遗传操作诱导杏鲍菇单核子实体发育。基因表达分析揭示了参与杏鲍鱼子实体发育的关键基因和信号通路。
Fruiting body formation is the most important developmental event in the edible mushroom life cycle; however, the genetic regulation of this process is not well understood. Pleurotus eryngii is a widely cultivated mushroom with high economic value. The mating of two
monokaryons carrying compatible A and B mating-type genes is required for the development of fruiting bodies in P. eryngii. In this study, we showed that the
monokaryons of P. eryngii transformed with compatible homeodomain (A mating type) and pheromone (B mating type) genes can complete fruiting body development but cannot form basidiospores. Transcriptional analyses revealed that expression of endogenous homeodomain and pheromone receptor genes and mating signaling pathways were activated by transferred homeodomain and pheromone genes in the transformants. Our findings provide a novel model for studying fruiting body development, which may accelerate the genetic breeding of edible mushrooms in the future. IMPORTANCE Fruiting bodies of edible mushrooms have high nutritional value. However, the fruiting body development of mushrooms is not well understood, and thus, many wild edible mushrooms of economic importance cannot be cultivated artificially. Moreover, variety among cultivatable mushrooms has improved marginally. Under natural conditions, fruiting body development can be initiated only in a dikaryon, the sexual mycelium obtained from mating two compatible
monokaryons. The present work showed induction of fruiting body development in Pleurotus eryngii
monokaryons by genetic manipulation. Gene expression analyses revealed key genes and signaling pathways involved in the fruiting body development of P. eryngii.