这项研究调查了杀虫剂多角菌对模型生物Bombyxmori(Linnaeus)的毒性作用,并探讨了O-香兰素的潜在改善特性。通过口服给家蚕幼虫给予亚致死浓度的多杀菌素,导致体重下降,幼虫长度,和受损的茧特征。对酶促和非酶促抗氧化剂的研究揭示了肠道中的氧化应激,脂肪的身体,和丝腺组织,以抗氧化剂减少和脂质过氧化增加为特征。然而,用O-香兰素治疗后有效地减轻了这些毒性作用,保持抗氧化能力和防止脂质过氧化。此外,O-香兰素可防止体重减轻并改善茧特性。在组织学层面,多杀菌素暴露导致肠道轻度组织学损伤,脂肪的身体,和丝腺。然而,O-香兰素后处理具有改善作用并减轻组织学损害。为了深入研究O-香兰素的改善机制,在计算机研究被用来研究家蚕的一个重要的异生代谢蛋白之间的相互作用,即,细胞色素p450,特别是CYP9A19和O-香兰素。我们进行了盲分子对接,然后进行了分子动力学模拟,结果表明,O-香兰素与家蚕细胞色素P450蛋白CYP9A19之间稳定的结合相互作用,属于CYP9A亚家族,提示O-香兰素在调节异种生物代谢中的潜在作用。
This study investigates the toxic effects of the insecticide spinetoram on the model organism Bombyx mori (Linnaeus) and explores the potential ameliorative properties of O-Vanillin. Sub-lethal concentrations of spinetoram were given to silkworm larvae via oral feed, resulting in reduced body weight, larval length, and impaired cocoon characteristics. A study of the enzymatic and non-enzymatic antioxidants revealed oxidative stress in the gut, fat body, and silk gland tissues, characterized by decreased antioxidants and increased lipid peroxidation. However, post-treatment with O-Vanillin effectively mitigated these toxic effects, preserving antioxidant capacities and preventing lipid peroxidation. Additionally, O-Vanillin prevented the loss of body weight and improved cocoon characteristics. At the histological level, spinetoram exposure caused mild histological damage in the gut, fat body, and silk gland. However, O-Vanillin post-treatment had ameliorative effects and mitigated the histological damages. To delve deeper into the mechanism of amelioration of O-Vanillin, in silico studies were used to study the interaction between an important xenobiotic metabolism protein of the Bombyx mori, i.e., Cytochrome p450, specifically CYP9A19, and O-Vanillin. We performed blind molecular docking followed by molecular dynamic simulation, and the results demonstrated stable binding interactions between O-Vanillin and CYP9A19, a cytochrome P450 protein in silkworm, belonging to the subfamily CYP9A, suggesting a potential role for O-vanillin in modulating xenobiotic metabolism.