背景:在这项研究中,我们详细调查了大麻二酚(CBD)的作用,β-石竹烯(BC),或其组合在糖尿病周围神经病变(DN)中。导致DN的关键因素包括线粒体功能障碍,炎症,和氧化应激。
方法:简而言之,腹腔注射链脲佐菌素(STZ)(55mg/kg)诱导SD大鼠DN,我们执行了涉及兰德尔·塞利托卡钳的程序,VonFrey的美感仪,一个热板,和冷板法测定体内的机械和热痛觉过敏。使用激光多普勒设备评估流向神经的血流。施万细胞暴露于30mM剂量的高葡萄糖(HG)诱导高血糖和DCFDA,进行JC1和Mitosox染色以确定线粒体膜电位,活性氧,和体外线粒体超氧化物。大鼠给予BC(30mg/kg),CBD(15mg/kg),或通过腹膜内注射组合,而施万细胞用3.65μMCBD处理,75µMBC,或组合评估其在DN改善中的作用。
结果:我们的结果表明,暴露于BC和CBD可减少HG诱导的施万细胞高血糖,部分是通过降低线粒体膜电位,活性氧,和线粒体超氧化物.此外,体内BC和CBD联合治疗可以通过促进自噬和线粒体生物发生,同时改善血流量,从而防止线粒体质量控制系统的恶化。CBD和BC治疗也减少了痛觉过敏和异常性疼痛的敏感性,在糖尿病大鼠中具有增强的抗氧化和抗炎作用。这些体内效应归因于AMPK的显著上调,sirT3,Nrf2,PINK1,PARKIN,LC3B,Beclin1和TFAM函数,而NLRP3炎性体的下调,NFκB,使用Western印迹记录COX2和p62活性。
结论:本研究表明,STZ和HG诱导的氧化和硝化应激在糖尿病神经病变的发病机制中起着至关重要的作用。我们发现,第一次,CBD和BC组合通过调节线粒体质量控制系统来改善DN。
BACKGROUND: In this study, we investigated in detail the role of cannabidiol (CBD), beta-caryophyllene (BC), or their combinations in diabetic peripheral neuropathy (DN). The key factors that contribute to DN include mitochondrial dysfunction, inflammation, and oxidative stress.
METHODS: Briefly, streptozotocin (STZ) (55 mg/kg) was injected intraperitoneally to induce DN in Sprague-Dawley rats, and we performed procedures involving Randall Sellito calipers, a Von Frey aesthesiometer, a hot plate, and cold plate methods to determine mechanical and thermal hyperalgesia in vivo. The blood flow to the nerves was assessed using a laser Doppler device. Schwann cells were exposed to high glucose (HG) at a dose of 30 mM to induce hyperglycemia and DCFDA, and JC1 and Mitosox staining were performed to determine mitochondrial membrane potential, reactive oxygen species, and mitochondrial superoxides in vitro. The rats were administered BC (30 mg/kg), CBD (15 mg/kg), or combination via i.p. injections, while Schwann cells were treated with 3.65 µM CBD, 75 µM BC, or combination to assess their role in DN amelioration.
RESULTS: Our results revealed that exposure to BC and CBD diminished HG-induced hyperglycemia in Schwann cells, in part by reducing mitochondrial membrane potential, reactive oxygen species, and mitochondrial superoxides. Furthermore, the BC and CBD combination treatment in vivo could prevent the deterioration of the mitochondrial quality control system by promoting autophagy and mitochondrial biogenesis while improving blood flow. CBD and BC treatments also reduced pain hypersensitivity to hyperalgesia and allodynia, with increased antioxidant and anti-inflammatory action in diabetic rats. These in vivo effects were attributed to significant upregulation of AMPK, sirT3, Nrf2, PINK1, PARKIN, LC3B, Beclin1, and TFAM functions, while downregulation of NLRP3 inflammasome, NFκB, COX2, and p62 activity was noted using Western blotting.
CONCLUSIONS: the present study demonstrated that STZ and HG-induced oxidative and nitrosative stress play a crucial role in the pathogenesis of diabetic neuropathy. We find, for the first time, that a CBD and BC combination ameliorates DN by modulating the mitochondrial quality control system.