■天然药物作为替代和/或补充治疗来对抗疾病在公共卫生中非常重要。因此,本研究的目的是评估黄杨果皮甲醇提取物的体外抗真菌活性,纸浆,和种子对抗白色念珠菌。
■本研究是体外实验和横断面实验。在Sabouraud葡萄糖琼脂中制备8个重复,每个5个孔,其中0.12%氯己定,蒸馏水,和种子的甲醇提取物,果皮,和巴林达果肉分别以10,690、8,270和6,430mg/mL的浓度放置,分别,根据Duraffourd量表评估敏感性。此外,通过稀释和琼脂接种法测定最小抑菌浓度(MIC)和最小杀菌浓度(MFC)。通过方差分析(ANOVA)和Tukey的事后检验进行统计分析,考虑P<0.05的显著性水平。
■种子巴林达甲醇提取物的抑制光晕,果皮,和纸浆对白色念珠菌的平均测量为15.94、11.94和11.56毫米,分别。种子的MIC,果皮,和纸浆提取物分别为1366.25、2067.5和1607.5mg/mL,而种子的MFC,果皮,和纸浆提取物分别为2672.50、2067.5和3215mg/mL,分别。此外,种子提取物的抗真菌活性明显高于果皮和果肉(P<0.001)。
■贝林达果皮甲醇提取物,纸浆,种子对白色念珠菌具有抑菌和杀真菌作用,对种子提取物非常敏感,MIC为1366.25mg/mL,MFC为2672.5mg/mL,这允许建议开发有效的药物配方来控制念珠菌病。
UNASSIGNED: Natural medicine used as an alternative and/or complementary treatment to counteract diseases is of great importance in public health. Therefore, the purpose of the present study was to assess the in vitro antifungal activity of Morinda citrifolia methanolic extract of peel, pulp, and seed against Candida albicans.
UNASSIGNED: The present study was experimental in vitro and cross-sectional. Eight replicates were prepared in Sabouraud dextrose agar with five wells each, where 0.12% chlorhexidine, distilled water, and methanolic extract of seed, peel, and pulp of Morinda citrifolia fruit were placed at concentrations of 10,690, 8,270, and 6,430 mg/mL, respectively, to evaluate sensitivity according to Duraffourd\'s scale. In addition, the minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) were determined by dilution and agar seeding method. Statistical analysis was performed by analysis of variance (ANOVA) and Tukey\'s post hoc test, considering a significance level of P < 0.05.
UNASSIGNED: The inhibition halos of Morinda citrifolia methanolic extract of seed, peel, and pulp against Candida albicans measured on average 15.94, 11.94, and 11.56 mm, respectively. The MIC of seed, peel, and pulp extract were 1366.25, 2067.5, and 1607.5 mg/mL respectively, whereas the MFC for seed, peel, and pulp extract were 2672.50, 2067.5, and 3215 mg/mL, respectively. Moreover, seed extract presented significantly higher antifungal activity than peel and pulp (P < 0.001).
UNASSIGNED: Morinda citrifolia methanolic extract of peel, pulp, and seed showed fungistatic and fungicidal effect against Candida albicans, being this very sensitive to seed extract with a MIC of 1366.25 mg/mL and a MFC of 2672.5 mg/mL, which allows recommending the development of effective pharmacological formulations for the control of candidiasis.