细小的灰尘会导致各种疾病,包括心血管,神经学,肾,生殖,电机,系统性,呼吸,和癌症疾病。因此,研究功能材料以防止这些问题是必要的。本研究使用miRNA分析等方法研究了芥酸对细尘的有益作用,定量PCR,流式细胞术,ELISA,和茜素O染色。芥酸可有效抑制炎症并上调暴露于细尘的成纤维细胞中的成骨激活剂。此外,芥酸诱导的外泌体(EIE)强烈抵消细粉尘对骨细胞分化和炎症的负面影响。尽管暴露在细尘中,EIE促进脂肪干细胞(ASCs)的成骨细胞分化,并增强巨噬细胞的成骨和吞噬作用。EIE对RunX2和BMP7的显着上调表明其在骨细胞分化和对微尘影响的保护中具有很强的作用。EIE还增强免疫活性并充当巨噬细胞的成骨触发器。MicroRNA分析显示EIE显著上调miRNAs,包括hsa-miRNA-1301-3p,hsa-miRNA-1908-5p,hsa-miRNA-423-5p,和hsa-miRNA-122-5p,与成骨分化和免疫力有关。因此,EIE显示出作为预防环境传播疾病的生物材料的潜力。
Fine dust causes various disorders, including cardiovascular, neurological, renal, reproductive, motor, systemic, respiratory, and cancerous diseases. Therefore, it is essential to study functional materials to prevent these issues. This study investigated the beneficial effects of erucic acid against fine dust using methods such as miRNA profiling, quantitative PCR, flow cytometry, ELISA, and Alizarin O staining. Erucic acid effectively suppresses inflammation and upregulates osteogenic activators in fibroblasts exposed to fine dust. Additionally, erucic acid-induced exosomes (EIEs) strongly counteract the negative effects of fine dust on osteocytic differentiation and inflammation. Despite fine dust exposure, EIEs promoted osteocytic differentiation in adipose-derived stem cells (ASCs) and enhanced osteogenesis and phagocytosis in macrophages. The significant upregulation of RunX2 and BMP7 by EIEs indicates its strong role in osteocytic differentiation and protection against the effects of fine dust. EIEs also boosts immune activity and acts as an osteogenic trigger for macrophages. MicroRNA profiling revealed that EIEs dramatically upregulated miRNAs, including hsa-miRNA-1301-3p, hsa-miRNA-1908-5p, hsa-miRNA-423-5p, and hsa-miRNA-122-5p, which are associated with osteogenic differentiation and immunity. Therefore, EIEs show potential as biomaterials to prevent environment-borne diseases.