■脊髓型颈椎病(CSM),全球脊髓功能障碍最常见的原因,是一种导致非暴力的退行性疾病,渐进的,和颈脊髓的长期压迫。这项研究的目的是研究微血管增殖是否可以积极影响实验性脊髓型颈椎病(CSM)的神经功能恢复。
■将60名成年雄性Sprague-Dawley(SD)随机分为四组:对照组(CON),压缩(COM),血管停滞(AS),和血管生成(AG),每组15只大鼠。AS组大鼠接受SU5416抑制血管生成,AG组大鼠接受去铁胺(DFO)促进血管生成。使用BassoBeattieBresnahan(BBB)量表和体感诱发电位(SEP)检查评估运动和感觉功能。通过神经元的数量来评估神经病理学变性,Nissl体(NB),苏木精和伊红(HE)检测到白质的去髓鞘化,甲苯胺蓝(TB),和Luxol固蓝(LFB)染色。免疫组织化学(IHC)染色用于观察神经血管单元(NVU)。
■CON组的大鼠表现出正常的运动功能,具有完整的BBB评分,正常的SEP潜伏期和振幅。在其他三组中,AG组BBB评分最高,SEP潜伏期最短,AS组BBB评分最低,SEP潜伏期最长。SEP幅度显示出与延迟相反的性能。与COM和AS组相比,AG组表现出明显的灰质神经元恢复和白质轴突髓鞘再生。DFO促进微血管增殖,尤其是在灰质中,并提高了神经胶质细胞的存活率。相比之下,SU-5416通过减少微血管抑制神经胶质细胞的活力。
■微血管状态与NVU重塑和功能恢复密切相关。因此,微血管的增殖有助于实验性CSM的功能恢复,这可能与NVU重塑相关联。
UNASSIGNED: Cervical Spondylotic Myelopathy (CSM), the most common cause of spinal cord dysfunction globally, is a degenerative disease that results in non-violent, gradual, and long-lasting compression of the cervical spinal cord. The objective of this study was to investigate whether microvascular proliferation could positively affect neural function recovery in experimental cervical spondylotic myelopathy (CSM).
UNASSIGNED: A total of 60 male adult Sprague-Dawley (SD) were randomly divided into four groups: Control (CON), Compression (COM), Angiostasis (AS), and Angiogenesis (A G),with 15 rats in each group. Rats in the AS group received SU5416 to inhibit angiogenesis, while rats in the AG group received Deferoxamine (DFO) to promote angiogenesis. Motor and sensory functions were assessed using the Basso Beattie Bresnahan (BBB) scale and somatosensory evoked potential (SEP) examination. Neuropathological degeneration was evaluated by the number of neurons, Nissl bodies (NB), and the de-myelination of white matter detected by Hematoxylin & Eosin(HE), Toluidine Blue (TB), and Luxol Fast Blue (LFB) staining. Immunohistochemical (IHC) staining was used to observe the Neurovascular Unit (NVU).
UNASSIGNED: Rats in the CON group exhibited normal locomotor function with full BBB score, normal SEP latency and amplitude. Among the other three groups, the AG group had the highest BBB score and the shortest SEP latency, while the AS group had the lowest BBB score and the most prolonged SEP latency. The SEP amplitude showed an opposite performance to the latency. Compared to the COM and AS groups, the AG group demonstrated significant neuronal restoration in gray matter and axonal remyelination in white matter. DFO promoted microvascular proliferation, especially in gray matter, and improved the survival of neuroglial cells. In contrast, SU-5416 inhibited the viability of neuroglial cells by reducing micro vessels.
UNASSIGNED: The microvascular status was closely related to NVU remodeling an-d functional recovery. Therefore, proliferation of micro vessels contributed to function -al recovery in experimental CSM, which may be associated with NVU remodeling.