RosalaxaRetz.是新疆一种尚未开发的玫瑰科植物,中国,它的花传统上在哈萨克族用来治疗普通感冒,发烧,和癫痫发作,减轻衰老的影响。在本研究中,生药学资料,物理化学性质,植物化学特征,和体外抗氧化效力的罗莎laxaRetz。提出了Flos(RLF)。在RLF的生药学评估中,感官特征,内部结构,观察到粉末信息,和物理化学参数,包括水分含量,灰,pH值,肿胀程度,和提取物进行了检查。对4种不同极性RLF提取物的化学成分进行定量分析,结果表明,水性部分的总三萜酸含量最高,类黄酮,和多酚含量(4.50±0.04mg/g,50.56±0.03mg/g,和60.20±0.09毫克/克,分别)。建立了高效液相色谱(HPLC)-二极管阵列检测器(DAD)方法,鞣花酸,黄芪,同时测定RLF中的利洛苷。在设定的浓度范围内,四种成分之间的线性关系良好(r>0.999),平均回收率为97.36%~100.54%。没食子酸的含量,鞣花酸,黄芪,RLF样品中的tiliroside为(9.46±2.31)mg/g,(10.60±0.75)mg/g,(1.13±2.50)mg/g,和(1.11±2.65)mg/g,分别。通过荧光测定其次级代谢产物的类型,化学溶剂法显色反应,和紫外可见(UV-Vis)光谱。通过傅里叶变换红外(FTIR)光谱法测定其次级代谢产物的官能团。结果表明,RLF含有多种次生代谢产物,包括类黄酮,酚酸,糖苷,有机酸。TLC鉴定显示它含有熊果酸,β-谷甾醇,利洛苷,黄芪,异槲皮苷,山奈酚-3-O-鲁丁苷,没食子酸,还有鞣花酸.通过DPPH研究了RLF不同极性部位的体外抗氧化活性,ABTS,和还原性能实验。水提物的抗氧化能力最强,与高含量的三萜酸一致,黄酮类化合物,和多酚化合物。这些发现将为研究RLF及其提取物的质量标准和药用价值提供关键信息,证明它在传统药用系统中的使用,并鼓励在疾病预防和治疗中使用这种植物。其植物化学成分和药理研究有待进一步探索。研究重点:采用光学显微镜和扫描电子显微镜(SEM)观察,和罗莎·莱卡·雷茨的微观结构。Flos(RLF)。物理化学性质,利用紫外-可见和红外光谱对4种不同极性RLF提取物的荧光和植物化学组成进行了分析。总三萜酸的测定,总黄酮,紫外分光光度法测定四种不同极性RLF提取物中的总多酚。建立了高效液相色谱(HPLC)-二极管阵列检测器(DAD)方法,鞣花酸,黄芪,同时测定RLF中的利洛苷。TLC证实RLF含有熊果酸,β-谷甾醇,利洛苷,黄芪,异槲皮苷,山奈酚3-鲁丁苷,没食子酸,还有鞣花酸.通过DPPH研究了RLF的体外抗氧化活性,ABTS,减少能力实验。
Rosa laxa Retz. is an unexplored Rosaceae plant in Xinjiang, China, and its flower is traditionally used in Kazak to treat the common cold, fever, and epileptic seizures and lessen the effects of aging. In the present study, the pharmacognostic profiles, physicochemical properties, phytochemical characteristics, and in vitro antioxidant potency of Rosa laxa Retz. flos (RLF) were presented. In the pharmacognostic evaluation of RLF, organoleptic characteristics, internal structures, and powder information were observed, and physicochemical parameters, including moisture content, ash, pH value, swelling degree, and extractives were examined. The quantitative analysis of the chemical composition of four different polar extracts of RLF showed that the aqueous part had the highest total triterpene acid, flavonoid, and polyphenol content (4.50 ± 0.04 mg/g, 50.56 ± 0.03 mg/g, and 60.20 ± 0.09 mg/g, respectively). A high-performance liquid chromatography (HPLC)-diode array detector (DAD) method was established and the contents of gallic acid, ellagic acid, astragalin, and tiliroside in RLF were determined simultaneously. In the set concentration range, the linear relationship among the four components was good (r > 0.999), the average recoveries were 97.36%-100.54%. The contents of gallic acid, ellagic acid, astragalin, and tiliroside in RLF samples were (9.46 ± 2.31) mg/g, (10.60 ±0.75) mg/g, (1.13 ± 2.50) mg/g, and (1.11 ± 2.65) mg/g, respectively. The types of its secondary metabolites were determined by fluorescence, color reaction by chemical solvent method, and ultraviolet-visible (UV-Vis) spectroscopy. The functional groups of its secondary metabolites were determined by Fourier transform infrared (FTIR) spectroscopy. Results showed that RLF contains a variety of secondary metabolic products, including flavonoids, phenolic acid, glycoside, and organic acid. TLC identification showed it contains ursolic acid, β-sitosterol, tiliroside, astragalin, isoquercitrin, kaempferol-3-O-rutinoside, gallic acid, and ellagic acid. The in vitro antioxidant activity of different polar parts of RLF was investigated by DPPH, ABTS, and reduction performance experiments. The aqueous extract had the strongest antioxidant capacity, consistent with the high content of triterpene acids, flavonoids, and polyphenolic compounds. These findings will provide critical information for the study of quality standards and medicinal value of RLF and its extracts, justify its usage in traditional medicinal systems, and encourage the use of this plant in disease prevention and treatment. Its phytochemical composition and pharmacological studies need to be explored in future. RESEARCH HIGHLIGHTS: Optical
microscope and scanning electron
microscope (SEM) were used to observe the morphology, and microstructure of Rosa laxa Retz. flos (RLF). The physicochemical properties, fluorescence and phytochemical composition of four different polar extracts of RLF were analyzed by UV-Vis and FTIR. Determination of total triterpenic acid, total flavonoids, and total polyphenols in four different polar extracts of RLF by UV spectrophotometry. A high-performance liquid chromatography (HPLC)-diode array detector (DAD) method was established and the contents of gallic acid, ellagic acid, astragalin, and tiliroside in RLF were determined simultaneously. TLC confirmed that RLF contains ursolic acid, β-sitosterol, tiliroside, astragalin, isoquercitrin, kaempferol 3-rutinoside, gallic acid, and ellagic acid. The in vitro antioxidant activity of RLF was studied by DPPH, ABTS, and reducing ability experiments.