目的:暴露于砷和六价铬是一个主要的公共卫生问题,特别是在世界发展中地区,并且缺乏可靠的治疗模式的信息。正是在这方面,本研究评估了作为预处理剂对重铬酸钾(K2Cr2O7)和亚砷酸钠(NaAsO2)暴露时,催吐露木霉(MRV)的甲醇叶提取物的功效。
方法:将7至10周龄的瑞士白化病小鼠分成8组,每组5只动物。治疗组由蒸馏水对照组组成,单独MRV(每日275mg/kgpo),K2Cr2O7(12.0mg/kg,单IP注入)+/-MRV预处理,NaAsO2(2.5mg/kg,单IP注入)+/-MRV预处理,Na2AsO2+K2Cr2O7+/-MRV预处置。连续七天给予MRV,在实验的第7天注射K2Cr2O7和NaAsO2。在骨髓细胞中确定微核多色红细胞(mPCE)的频率,同时评估血浆中的天冬氨酸转氨酶(AST)和丙氨酸转氨酶(ALT)活性。肝谷胱甘肽(GSH),丙二醛(MDA),还测定了过氧化氢酶(CAT)和谷胱甘肽-S-转移酶(GST)水平。
结果:NaAsO2和K2Cr2O7显著(p<0.05)增加了mPCE的形成,AST,ALT,和CAT与对照相比。同时暴露于NaAsO2和K2Cr2O7进一步增加了标记物的水平。此外,NaAsO2或K2Cr2O7或其组合可显著降低GSH和GST。用MRV预处理使标记物向对照逆转。
结论:因此,催吐草的甲醇提取物可能通过降低氧化应激和强化抗氧化系统来改善NaAsO2和K2Cr2O7诱导的毒性。
OBJECTIVE: Exposure to arsenic and hexavalent chromium is a major public health concern especially in the developing part of the world and there is paucity of information on reliable treatment modalilities. It is in this regard that this study evaluates the efficacy of methanol leaf extract of Rauvolfia vomitoria (MRV) when used as pretreatment agent against potassium dichromate (K2Cr2O7) and sodium arsenite (NaAsO2) exposure.
METHODS: Swiss albino mice between 7 and 10 weeks old were divided into eight cohorts of five animals each. Treatment groups consisted of a distilled water control, MRV alone (275 mg/kg po daily), K2Cr2O7 (12.0 mg/kg, single ip injection) +/- MRV pretreatment, NaAsO2 (2.5 mg/kg, single ip injection) +/- MRV pretreatment, Na2AsO2 + K2Cr2O7 +/- MRV pretreatment. MRV was given for seven consecutive days, while K2Cr2O7 and NaAsO2 were injected on day seven of the experiment. The frequency of micronucleated polychromatic erythrocytes (mPCEs) was determined in bone marrow cells, while aspartate aminotransferase (AST) and alanine aminotransferase (ALT) activities were assessed in the plasma. Hepatic glutathione (GSH), malondialdehyde (MDA), catalase (CAT) and glutathione-S-transferase (GST) levels were also determined.
RESULTS: The NaAsO2 and K2Cr2O7 significantly (p<0.05) increased mPCE formation, AST, ALT, and CAT when compared with the control. Simultaneous exposure to NaAsO2 and K2Cr2O7 further increased the levels of the markers. Furthermore, GSH and GST were significantly reduced by NaAsO2 or K2Cr2O7 or their combination. Pretreatment with MRV reversed the markers towards that of control.
CONCLUSIONS: Methanol extract of Rauvolfia vomitoria may therefore ameliorate NaAsO2 and K2Cr2O7-induced toxicities via reduction of oxidative stress and fortification of anti-oxidant system.